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Literature summary for 1.14.18.3 extracted from

  • Miyaji, A.
    Particulate methane monooxygenase from Methylosinus trichosporium OB3b (2011), Methods Enzymol., 495, 211-225.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method Methylosinus trichosporium

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Methylosinus trichosporium 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ pmMO contains copper ions (150 nmol per mg protein in membrane fractions) that are required for its enzymatic activity. Some increase in pMMO activity is observed by adding CuSO4 Methylosinus trichosporium
Fe2+ pmMO contains 450 nmol Fe2+ per mg protein in membrane fractions Methylosinus trichosporium
additional information increase of activity is not observed by adding FeSO4 Methylosinus trichosporium
Zn2+ pmMO contains 1.5 nmol Zn2+ per mg protein in membrane fractions Methylosinus trichosporium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
1 * 40000 + 1 * 24000 + 1 * 21000, SDS-PAGE Methylosinus trichosporium
24000
-
1 * 40000 + 1 * 24000 + 1 * 21000, SDS-PAGE Methylosinus trichosporium
40000
-
1 * 40000 + 1 * 24000 + 1 * 21000, SDS-PAGE Methylosinus trichosporium

Organism

Organism UniProt Comment Textmining
Methylosinus trichosporium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
POROS 20HQ column chromatography, gel filtration. Purified pMMO is inherently instable in vitro Methylosinus trichosporium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.011
-
crude extract, using propylene as substrate, in 25 mM MOPS buffer (pH 7.0), at 30°C Methylosinus trichosporium
0.012
-
purified enzyme, using propylene as substrate, in 25 mM MOPS buffer (pH 7.0), at 30°C Methylosinus trichosporium

Storage Stability

Storage Stability Organism
4°C, pMMO in membrane fractions under nitrogen atmosphere, 4 days, 20% loss of activity Methylosinus trichosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3-butadiene + duroquinol + O2
-
Methylosinus trichosporium 1,2-epoxybut-3-ene + duroquinone + H2O 100% 1,2-epoxybut-3-ene is produced, 36% (R)-selectivity, 64% (S)-selectivity ?
1-bromopropane + duroquinol + O2
-
Methylosinus trichosporium 1-bromo-2-propanol + 1-propanol + 1-bromo-3-propanol + duroquinone + H2O 72% 1-bromo-2-propanol (70% (R)-selectivity, 30% (S)-selectivity), 24% 1-propanol and 4% 1-bromo-3-propanol are produced ?
1-bromopropene + duroquinol + O2
-
Methylosinus trichosporium 1-bromo-2,3-epoxypropane + allyl-alcohol + 1-propanol + duroquinone + H2O 63% 1-bromo-2,3-epoxypropane, 31% allyl-alcohol and 6% 1-propanol are produced ?
1-butene + duroquinol + O2
-
Methylosinus trichosporium 1,2-epoxybutane + duroquinone + H2O 100% epoxybutane is produced, 36% (R)-selectivity, 64% (S)-selectivity ?
1-chloropropane + duroquinol + O2
-
Methylosinus trichosporium 1-chloro-2-propanol + 1-propanol + 1-chloro-3-propanol + duroquinone + H2O 64% 1-chloro-2-propanol (70% (R)-selectivity, 30% (S)-selectivity), 29% 1-propanol and 7% 1-chloro-3-propanol are produced ?
1-chloropropene + duroquinol + O2
-
Methylosinus trichosporium 1-chloro-2,3-epoxypropane + allyl-alcohol + 1-propanol + duroquinone + H2O 67% 1-chloro-2,3-epoxypropane, 23% allyl-alcohol and 10% 1-propanol are produced ?
2-bromopropane + duroquinol + O2
-
Methylosinus trichosporium 2-bromo-1-propanol + acetone + duroquinone + H2O 2-bromo-1-propanol shows 26% (R)-selectivity and 74% (S)-selectivity ?
2-chloropropane + duroquinol + O2
-
Methylosinus trichosporium 2-chloro-1-propanol + ? + duroquinone + H2O 1-chloro-2-propanol shows 26% (R)-selectivity and 74% (S)-selectivity ?
butane + duroquinol + O2
-
Methylosinus trichosporium 2-butanol + butanal + duroquinone + H2O 91% 2-butanal and 9% butanal are produced ?
cis-2-butene + duroquinol + O2
-
Methylosinus trichosporium cis-2,3-epoxybutane + duroquinone + H2O cis-2,3-epoxybutane is produced ?
ethane + duroquinol + O2
-
Methylosinus trichosporium ethanal + duroquinone + H2O 100% ethanal is produced ?
ethylene + duroquinol + O2
-
Methylosinus trichosporium epoxyethane + duroquinone + H2O 100% epoxyethane produced ?
methane + duroquinol + O2
-
Methylosinus trichosporium methanol + duroquinone + H2O 100% methanol is produced ?
additional information decyl-plastoquinol, reduced coenzyme Q1, and trimethylquinol can drive pMMO, though its activity is lower than that with duroquinol. Succinate-driven pMMO activity in the membrane fractions is also observed Methylosinus trichosporium ?
-
?
pentane + duroquinol + O2
-
Methylosinus trichosporium 2-pentanol + pentanal + duroquinone + H2O 31% 2-pentanal and 69% pentanal are produced ?
propane + duroquinol + O2
-
Methylosinus trichosporium 2-propanol + propanal + duroquinone + H2O 84% 2-propanal and 16% propanal are produced ?
propene + duroquinol + O2
-
Methylosinus trichosporium 1,2-epoxypropane + duroquinone + H2O 57% (R)-selectivity, 43% (S)-selectivity ?
propylene + duroquinol + O2
-
Methylosinus trichosporium epoxypropane + allyl-alcohol + 1-propanol + duroquinone + H2O 95% epoxypropane, 4.6% allyl-alcohol and 0.4% butanal are produced ?
trans-2-butene + duroquinol + O2
-
Methylosinus trichosporium trans-2,3-epoxybutane + trans-2-butane-1-al + duroquinone + H2O 41% trans-2,3-epoxybutane and 59% trans-2-butane-1-al are produced ?

Subunits

Subunits Comment Organism
heterotrimer 1 * 40000 + 1 * 24000 + 1 * 21000, SDS-PAGE Methylosinus trichosporium

Synonyms

Synonyms Comment Organism
particulate methane monooxygenase
-
Methylosinus trichosporium
pMMO
-
Methylosinus trichosporium