Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method | Methylosinus trichosporium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Methylosinus trichosporium | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | pmMO contains copper ions (150 nmol per mg protein in membrane fractions) that are required for its enzymatic activity. Some increase in pMMO activity is observed by adding CuSO4 | Methylosinus trichosporium | |
Fe2+ | pmMO contains 450 nmol Fe2+ per mg protein in membrane fractions | Methylosinus trichosporium | |
additional information | increase of activity is not observed by adding FeSO4 | Methylosinus trichosporium | |
Zn2+ | pmMO contains 1.5 nmol Zn2+ per mg protein in membrane fractions | Methylosinus trichosporium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
1 * 40000 + 1 * 24000 + 1 * 21000, SDS-PAGE | Methylosinus trichosporium |
24000 | - |
1 * 40000 + 1 * 24000 + 1 * 21000, SDS-PAGE | Methylosinus trichosporium |
40000 | - |
1 * 40000 + 1 * 24000 + 1 * 21000, SDS-PAGE | Methylosinus trichosporium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylosinus trichosporium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
POROS 20HQ column chromatography, gel filtration. Purified pMMO is inherently instable in vitro | Methylosinus trichosporium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.011 | - |
crude extract, using propylene as substrate, in 25 mM MOPS buffer (pH 7.0), at 30°C | Methylosinus trichosporium |
0.012 | - |
purified enzyme, using propylene as substrate, in 25 mM MOPS buffer (pH 7.0), at 30°C | Methylosinus trichosporium |
Storage Stability | Organism |
---|---|
4°C, pMMO in membrane fractions under nitrogen atmosphere, 4 days, 20% loss of activity | Methylosinus trichosporium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3-butadiene + duroquinol + O2 | - |
Methylosinus trichosporium | 1,2-epoxybut-3-ene + duroquinone + H2O | 100% 1,2-epoxybut-3-ene is produced, 36% (R)-selectivity, 64% (S)-selectivity | ? | |
1-bromopropane + duroquinol + O2 | - |
Methylosinus trichosporium | 1-bromo-2-propanol + 1-propanol + 1-bromo-3-propanol + duroquinone + H2O | 72% 1-bromo-2-propanol (70% (R)-selectivity, 30% (S)-selectivity), 24% 1-propanol and 4% 1-bromo-3-propanol are produced | ? | |
1-bromopropene + duroquinol + O2 | - |
Methylosinus trichosporium | 1-bromo-2,3-epoxypropane + allyl-alcohol + 1-propanol + duroquinone + H2O | 63% 1-bromo-2,3-epoxypropane, 31% allyl-alcohol and 6% 1-propanol are produced | ? | |
1-butene + duroquinol + O2 | - |
Methylosinus trichosporium | 1,2-epoxybutane + duroquinone + H2O | 100% epoxybutane is produced, 36% (R)-selectivity, 64% (S)-selectivity | ? | |
1-chloropropane + duroquinol + O2 | - |
Methylosinus trichosporium | 1-chloro-2-propanol + 1-propanol + 1-chloro-3-propanol + duroquinone + H2O | 64% 1-chloro-2-propanol (70% (R)-selectivity, 30% (S)-selectivity), 29% 1-propanol and 7% 1-chloro-3-propanol are produced | ? | |
1-chloropropene + duroquinol + O2 | - |
Methylosinus trichosporium | 1-chloro-2,3-epoxypropane + allyl-alcohol + 1-propanol + duroquinone + H2O | 67% 1-chloro-2,3-epoxypropane, 23% allyl-alcohol and 10% 1-propanol are produced | ? | |
2-bromopropane + duroquinol + O2 | - |
Methylosinus trichosporium | 2-bromo-1-propanol + acetone + duroquinone + H2O | 2-bromo-1-propanol shows 26% (R)-selectivity and 74% (S)-selectivity | ? | |
2-chloropropane + duroquinol + O2 | - |
Methylosinus trichosporium | 2-chloro-1-propanol + ? + duroquinone + H2O | 1-chloro-2-propanol shows 26% (R)-selectivity and 74% (S)-selectivity | ? | |
butane + duroquinol + O2 | - |
Methylosinus trichosporium | 2-butanol + butanal + duroquinone + H2O | 91% 2-butanal and 9% butanal are produced | ? | |
cis-2-butene + duroquinol + O2 | - |
Methylosinus trichosporium | cis-2,3-epoxybutane + duroquinone + H2O | cis-2,3-epoxybutane is produced | ? | |
ethane + duroquinol + O2 | - |
Methylosinus trichosporium | ethanal + duroquinone + H2O | 100% ethanal is produced | ? | |
ethylene + duroquinol + O2 | - |
Methylosinus trichosporium | epoxyethane + duroquinone + H2O | 100% epoxyethane produced | ? | |
methane + duroquinol + O2 | - |
Methylosinus trichosporium | methanol + duroquinone + H2O | 100% methanol is produced | ? | |
additional information | decyl-plastoquinol, reduced coenzyme Q1, and trimethylquinol can drive pMMO, though its activity is lower than that with duroquinol. Succinate-driven pMMO activity in the membrane fractions is also observed | Methylosinus trichosporium | ? | - |
? | |
pentane + duroquinol + O2 | - |
Methylosinus trichosporium | 2-pentanol + pentanal + duroquinone + H2O | 31% 2-pentanal and 69% pentanal are produced | ? | |
propane + duroquinol + O2 | - |
Methylosinus trichosporium | 2-propanol + propanal + duroquinone + H2O | 84% 2-propanal and 16% propanal are produced | ? | |
propene + duroquinol + O2 | - |
Methylosinus trichosporium | 1,2-epoxypropane + duroquinone + H2O | 57% (R)-selectivity, 43% (S)-selectivity | ? | |
propylene + duroquinol + O2 | - |
Methylosinus trichosporium | epoxypropane + allyl-alcohol + 1-propanol + duroquinone + H2O | 95% epoxypropane, 4.6% allyl-alcohol and 0.4% butanal are produced | ? | |
trans-2-butene + duroquinol + O2 | - |
Methylosinus trichosporium | trans-2,3-epoxybutane + trans-2-butane-1-al + duroquinone + H2O | 41% trans-2,3-epoxybutane and 59% trans-2-butane-1-al are produced | ? |
Subunits | Comment | Organism |
---|---|---|
heterotrimer | 1 * 40000 + 1 * 24000 + 1 * 21000, SDS-PAGE | Methylosinus trichosporium |
Synonyms | Comment | Organism |
---|---|---|
particulate methane monooxygenase | - |
Methylosinus trichosporium |
pMMO | - |
Methylosinus trichosporium |