Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-amino-2-n-pentylbenzothiazole | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Saccharomyces cerevisiae | 16020 | - |
microsome | - |
Saccharomyces cerevisiae | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2 | Saccharomyces cerevisiae | step 3 of 3 of the overall reaction | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O | - |
? | |
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2 | Saccharomyces cerevisiae FL530 | step 3 of 3 of the overall reaction | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O | - |
? | |
4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | Saccharomyces cerevisiae | step 1 of 3 of the overall reaction | 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O | - |
? | |
4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | Saccharomyces cerevisiae FL530 | step 1 of 3 of the overall reaction | 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O | - |
? | |
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | Saccharomyces cerevisiae | step 2 of 3 of the overall reaction | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O | - |
? | |
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | Saccharomyces cerevisiae FL530 | step 2 of 3 of the overall reaction | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O | - |
? | |
additional information | Saccharomyces cerevisiae | enzyme plays a key role in sterol biosynthesis as component of the sterol-4-demethylation multienzyme complex, sterol metabolites analysis, overview | ? | - |
? | |
additional information | Saccharomyces cerevisiae FL530 | enzyme plays a key role in sterol biosynthesis as component of the sterol-4-demethylation multienzyme complex, sterol metabolites analysis, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
enzyme is a component of the sterol-4-demethylation multienzyme complex | - |
Saccharomyces cerevisiae FL530 | - |
enzyme is a component of the sterol-4-demethylation multienzyme complex | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
development of an enzyme assay for specific measurement of enzyme activity using [14C]-4,4-dimethyl-zymosterol as substrate | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2 | step 3 of 3 of the overall reaction | Saccharomyces cerevisiae | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O | - |
? | |
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2 | step 3 of 3 of the overall reaction | Saccharomyces cerevisiae FL530 | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O | - |
? | |
4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 1 of 3 of the overall reaction | Saccharomyces cerevisiae | 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O | - |
? | |
4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 1 of 3 of the overall reaction | Saccharomyces cerevisiae FL530 | 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O | - |
? | |
4,4-dimethyl-zymosterol + reduced acceptor + O2 | artificial substrate | Saccharomyces cerevisiae | ? | - |
? | |
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 2 of 3 of the overall reaction | Saccharomyces cerevisiae | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O | - |
? | |
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 2 of 3 of the overall reaction | Saccharomyces cerevisiae FL530 | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O | - |
? | |
additional information | enzyme plays a key role in sterol biosynthesis as component of the sterol-4-demethylation multienzyme complex, sterol metabolites analysis, overview | Saccharomyces cerevisiae | ? | - |
? | |
additional information | substrate specificity, 24-methylene-24-dihydrocycloartenol is a poor substrate, no activity with 24-ethylidene-24-dihydrolophenol, overview | Saccharomyces cerevisiae | ? | - |
? | |
additional information | enzyme plays a key role in sterol biosynthesis as component of the sterol-4-demethylation multienzyme complex, sterol metabolites analysis, overview | Saccharomyces cerevisiae FL530 | ? | - |
? | |
additional information | substrate specificity, 24-methylene-24-dihydrocycloartenol is a poor substrate, no activity with 24-ethylidene-24-dihydrolophenol, overview | Saccharomyces cerevisiae FL530 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme is a component of the sterol-4-demethylation multienzyme complex | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
4,4-dimethyl-sterol 4alpha-methyl-oxidase | - |
Saccharomyces cerevisiae |
4,4-dimethyl-zymosterol 4alpha-methyl-oxidase | - |
Saccharomyces cerevisiae |
SMO | - |
Saccharomyces cerevisiae |
sterol-C4-methyl-oxidase | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Saccharomyces cerevisiae |