Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cytochrome b5 reductase | NADH-specific, required, EC 1.6.2.2 | Rattus norvegicus |
General Stability | Organism |
---|---|
rapid isolation of enzyme and storage at -70°C is required | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CN- | complete and reversible inhibition at 0.5 mM | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | contains one atom of non-heme iron per enzyme molecule, catalytic function | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
x * 53000, disc-gel electrophoresis | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
stearoyl-CoA + AH2 + O2 | Rattus norvegicus | - |
oleoyl-CoA + A + H2O | - |
? | |
stearoyl-CoA + AH2 + O2 | Rattus norvegicus Long-Evans | - |
oleoyl-CoA + A + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Long-Evans | - |
Rattus norvegicus Long-Evans | - |
Long-Evans | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phospholipoprotein | tightly bound phospholipid is removed during purification | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
preparation of 11 fractions, ion-exchange, gel filtration | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.24 | 0.36 | - |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
enzyme cannot be stored overnight without loss of 20-50% of activity before fraction 5 | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
stearoyl-CoA + AH2 + O2 | - |
Rattus norvegicus | oleoyl-CoA + A + H2O | - |
? | |
stearoyl-CoA + AH2 + O2 | - |
Rattus norvegicus Long-Evans | oleoyl-CoA + A + H2O | - |
? | |
stearoyl-CoA + NADH + O2 | terminal component in the enzyme system | Rattus norvegicus | oleoyl-CoA + NAD+ + H2O | - |
? | |
stearoyl-CoA + NADH + O2 | terminal component in the enzyme system | Rattus norvegicus Long-Evans | oleoyl-CoA + NAD+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53000, disc-gel electrophoresis | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
32 | - |
30 min, 70-80% of activity | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.35 | - |
stearoyl-CoA | 25°C | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome b5 | required | Rattus norvegicus | |
additional information | phospholipid is required | Rattus norvegicus | |
NADH | dependent | Rattus norvegicus |