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Literature summary for 1.14.19.19 extracted from

  • Tian, Y.; Zhao, G.Y.; Fang, W.; Xu, Q.; Tan, R.X.
    DELTA10(E)-Sphingolipid desaturase involved in fusaruside mycosynthesis and stress adaptation in Fusarium graminearum (2015), Sci. Rep., 5, 10486 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine the enzyme can be useful for production of fusaruside, a 10,11-unsaturated immunosuppressive fungal sphingolipid with medical potentials for treating liver injury and colitis Fusarium graminearum
synthesis the enzyme can be useful for production of fusaruside, a 10,11-unsaturated immunosuppressive fungal sphingolipid with medical potentials for treating liver injury and colitis, DELTA 10(E)-sphingolipid desaturase as a new regiospecific biocatalyst Fusarium graminearum

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His6-tagged wild-type and mutant enzymes in Pichia pastoris strain GS115 Fusarium graminearum

Protein Variants

Protein Variants Comment Organism
D281A site-directed mutagenesis, the expressed mutant enzyme is soluble but catalytically inactive Fusarium graminearum
H280A site-directed mutagenesis of a conserved His residue of the histidine box, the expressed mutant enzyme is soluble but catalytically inactive Fusarium graminearum
H284A site-directed mutagenesis of a conserved His residue of the histidine box, the expressed mutant enzyme is soluble but catalytically inactive Fusarium graminearum
H317A site-directed mutagenesis of a conserved His residue of the histidine box, the expressed mutant enzyme is soluble but catalytically inactive Fusarium graminearum
H320A site-directed mutagenesis of a conserved His residue of the histidine box, the expressed mutant enzyme is soluble but catalytically inactive Fusarium graminearum
H321A site-directed mutagenesis of a conserved His residue of the histidine box, the expressed mutant enzyme is soluble but catalytically inactive Fusarium graminearum
H504A site-directed mutagenesis of a conserved His residue of the histidine box, the expressed mutant enzyme is soluble but catalytically inactive Fusarium graminearum
H505A site-directed mutagenesis of a conserved His residue of the histidine box, the expressed mutant enzyme is soluble but catalytically inactive Fusarium graminearum
additional information knockout of the DELTA10(E)-SD gene abolishes fusaruside production, overview Fusarium graminearum
V502A site-directed mutagenesis, the expressed mutant enzyme is soluble and shows slightly reduced catalytic activity compared to the wild-type enzyme Fusarium graminearum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten steady-state kinetics Fusarium graminearum
0.00206
-
cerebroside B recombinant wild-type enzyme, pH 8.0, 28°C Fusarium graminearum

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Fusarium graminearum 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cerebroside B + 2 ferrocytochrome b5 + O2 + 2 H+ Fusarium graminearum a (4E,8E)-sphinga-4,8-dienine ceramide fusaruside + 2 ferricytochrome b5 + 2 H2O
-
?
cerebroside B + 2 ferrocytochrome b5 + O2 + 2 H+ Fusarium graminearum CBS123657 a (4E,8E)-sphinga-4,8-dienine ceramide fusaruside + 2 ferricytochrome b5 + 2 H2O
-
?

Organism

Organism UniProt Comment Textmining
Fusarium graminearum A0A016Q182
-
-
Fusarium graminearum CBS123657 A0A016Q182
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged wild-type and mutant enzymes from Pichia pastoris strain GS115 by nickel affinity chromatography Fusarium graminearum

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Fusarium graminearum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cerebroside B + 2 ferrocytochrome b5 + O2 + 2 H+ a (4E,8E)-sphinga-4,8-dienine ceramide Fusarium graminearum fusaruside + 2 ferricytochrome b5 + 2 H2O
-
?
cerebroside B + 2 ferrocytochrome b5 + O2 + 2 H+ a (4E,8E)-sphinga-4,8-dienine ceramide, high substrate Fusarium graminearum fusaruside + 2 ferricytochrome b5 + 2 H2O
-
?
cerebroside B + 2 ferrocytochrome b5 + O2 + 2 H+ a (4E,8E)-sphinga-4,8-dienine ceramide Fusarium graminearum CBS123657 fusaruside + 2 ferricytochrome b5 + 2 H2O
-
?
cerebroside B + 2 ferrocytochrome b5 + O2 + 2 H+ a (4E,8E)-sphinga-4,8-dienine ceramide, high substrate Fusarium graminearum CBS123657 fusaruside + 2 ferricytochrome b5 + 2 H2O
-
?
additional information LC-MS screenings of sphingolipid-enriched fractions and in vitro enzymatic synthesis of fusaruside by recombinant DELTA 10(E)-SD. The enzyme shows no reactivity towards 2-methylbutene-bearing molecules including citral, isochromophilone VI, nerolidol, phytolorvitamin D1 and cerebroside B derived ceramide. The presence of the C9-methyl group is essential for the sphingolipid DELTA 10-desaturase activity, mutational analysis Fusarium graminearum ?
-
?
additional information LC-MS screenings of sphingolipid-enriched fractions and in vitro enzymatic synthesis of fusaruside by recombinant DELTA 10(E)-SD. The enzyme shows no reactivity towards 2-methylbutene-bearing molecules including citral, isochromophilone VI, nerolidol, phytolorvitamin D1 and cerebroside B derived ceramide. The presence of the C9-methyl group is essential for the sphingolipid DELTA 10-desaturase activity, mutational analysis Fusarium graminearum CBS123657 ?
-
?

Synonyms

Synonyms Comment Organism
DELTA 10(E)-SD
-
Fusarium graminearum
DELTA 10(E)-sphingolipid desaturase
-
Fusarium graminearum
sphingolipid 10,11-desaturase
-
Fusarium graminearum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
assay at Fusarium graminearum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Fusarium graminearum

Cofactor

Cofactor Comment Organism Structure
cytochrome b5
-
Fusarium graminearum
additional information the enzyme assay is performed with 2 mM NADH Fusarium graminearum

General Information

General Information Comment Organism
metabolism the fusaruside biosynthetic pathway involves the enzyme, overview Fusarium graminearum
physiological function the enzyme catalyzes the regiospecific biosynthesis of fusaruside, a 10,11-unsaturated immunosuppressive fungal sphingolipid through desaturation of cerebroside B, an abundant fungal sphingolipid. The conserved histidines of DELTA 10(E)-SD are essential for the 10,11-desaturation catalysis, which is also preconditioned by the C9-methylation of the substrate. Enzyme DELTA 10(E)-SD improves survival of fungal strains in chill and salinity Fusarium graminearum