Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.19.29 extracted from

  • Li, S.; Zhang, G.; Yuan, Y.; Wang, C.; Gao, W.; Deng, C.; Lu, L.; Hu, Z.
    Three homologous genes encoding functional DELTA8-sphingolipid desaturase in Populus tomentosa (2014), Genes Genomics, 36, 293-301 .
No PubMed abstract available
Search for more literature for 1.14.19.29

Cloned(Commentary)

Cloned (Comment) Organism
gene PtD8A, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis, functional recombinant expression in Saccharomyces cerevisiae strain INVSc1 Populus tomentosa
gene PtD8B, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis, functional recombinant expression in Saccharomyces cerevisiae strain INVSc1 Populus tomentosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+ Populus tomentosa
-
 a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
 ?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+ Populus tomentosa
-
 a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
 ?
additional information Populus tomentosa the DELTA8-sphingolipid desaturase is able to catalyze desaturation at the C8 position of the long-chain base of sphingolipids in plants. The recombinant enzyme catalyzes the C18-phytosphingenine desaturation to produce 8(Z)- and 8(E)-C18-phytosphingenine ?
-
 ?

Organism

Organism UniProt Comment Textmining
Populus tomentosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant functional enzyme from Saccharomyces cerevisiae strain INVSc1 Populus tomentosa

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Populus tomentosa
-
leaf higher expression level Populus tomentosa
-
additional information PtD8A is expressed in all tissues analyzed. Expression level of PtD8C is clearly much lower than that of PtD8A and PtD8B Populus tomentosa
-
additional information PtD8B is expressed in all tissues analyzed. Expression level of PtD8C is clearly much lower than that of PtD8A and PtD8B Populus tomentosa
-
additional information PtD8C is not expressed in roots. Expression level of PtD8C is clearly much lower than that of PtD8A and PtD8B Populus tomentosa
-
root
-
 Populus tomentosa
-
stem
-
 Populus tomentosa
-
stem higher expression level Populus tomentosa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
-
 Populus tomentosa a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
 ?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
-
 Populus tomentosa a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
 ?
additional information the DELTA8-sphingolipid desaturase is able to catalyze desaturation at the C8 position of the long-chain base of sphingolipids in plants. The recombinant enzyme catalyzes the C18-phytosphingenine desaturation to produce 8(Z)- and 8(E)-C18-phytosphingenine Populus tomentosa ?
-
 ?
additional information isozyme PtD8A preferably forms (8E)-products, ratio 8(Z):8(E) is 0.25 Populus tomentosa ?
-
 ?
additional information isozyme PtD8B preferably forms (8E)-products, ratio 8(Z):8(E) is 0.17 Populus tomentosa ?
-
 ?
additional information isozyme PtD8C preferably forms (8Z)-products, ratio 8(Z):8(E) is 12.71 Populus tomentosa ?
-
 ?

Synonyms

Synonyms Comment Organism
DELTA8-sphingolipid desaturase
-
 Populus tomentosa
PtD8A
-
 Populus tomentosa
PtD8B
-
 Populus tomentosa
PtD8C
-
 Populus tomentosa

Cofactor

Cofactor Comment Organism Structure
cytochrome b5
-
 Populus tomentosa

General Information

General Information Comment Organism
physiological function the DELTA8-sphingolipid desaturase is able to catalyze desaturation at the C8 position of the long-chain base of sphingolipids in plants Populus tomentosa