Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae | Vernicia fordii |
gene FADX, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional expression of the enzyme in Saccharomyces cerevisiae strain MMYO11, transient expression of myc-tagged enzyme in Nicotiana tabacum cv. Bright-Yellow 2 suspension-cultured BY-2 cells | Vernicia fordii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Vernicia fordii | 5783 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ | Vernicia fordii | the enzyme is involved in the biosynthetic pathway of eleostearic acid | an alpha-parinaroyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O | alpha-parinarate = (9Z,11E,13E,15Z)-octadeca-9,11,13,15-tetraenoate | ? | |
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ | Vernicia fordii | - |
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O | - |
? | |
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ | Vernicia fordii | the enzyme is involved in the biosynthetic pathway of eleostearic acid | an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O | alpha-eleostearate = (9Z,11E,13E)-octadeca-9,11,13-trienoate | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vernicia fordii | Q8GZC2 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | expressed exclusively within developing seeds | Vernicia fordii | - |
seed | FADX is expressed exclusively in developing tung seeds | Vernicia fordii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(6Z,9Z,12Z)-octadeca-6,9,12-trienoate + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Vernicia fordii | (6Z,9Z,11E,13E)-octadeca-6,9,11,13-tetraenoate + 2 ferricytochrome b5 + 2 H2O | - |
? | |
(9Z)-hexadecenoate + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Vernicia fordii | (9Z,12E)-hexadeca-9,12-dienoate + 2 ferricytochrome b5 + 2 H2O | - |
? | |
(9Z)-octadecenoate + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Vernicia fordii | (9Z,12E)-octadeca-9,12-dienoate + 2 ferricytochrome b5 + 2 H2O | - |
? | |
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Vernicia fordii | an alpha-parinaroyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O | alpha-parinarate = (9Z,11E,13E,15Z)-octadeca-9,11,13,15-tetraenoate | ? | |
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ | the enzyme is involved in the biosynthetic pathway of eleostearic acid | Vernicia fordii | an alpha-parinaroyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O | alpha-parinarate = (9Z,11E,13E,15Z)-octadeca-9,11,13,15-tetraenoate | ? | |
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Vernicia fordii | an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O | - |
? | |
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Vernicia fordii | an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O | alpha-eleostearate = (9Z,11E,13E)-octadeca-9,11,13-trienoate | ? | |
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ | the enzyme is involved in the biosynthetic pathway of eleostearic acid | Vernicia fordii | an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O | alpha-eleostearate = (9Z,11E,13E)-octadeca-9,11,13-trienoate | ? | |
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ | the double bond introduced by FADX during fatty acid desaturation is in the trans, rather than cis, configuration | Vernicia fordii | an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O | - |
? | |
additional information | the enzyme is capable of generating a variety of alternative conjugated and 12-desaturated fatty acid products in yeast cells cultured in the presence of exogenously supplied fatty acid substrates | Vernicia fordii | ? | - |
? | |
additional information | a bifunctional enzyme also exhibiting the activity of EC 1.14.19.34 | Vernicia fordii | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DELTA12 desaturase | - |
Vernicia fordii |
DELTA12 oleate desaturase | - |
Vernicia fordii |
FADX | - |
Vernicia fordii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome b5 | - |
Vernicia fordii |
General Information | Comment | Organism |
---|---|---|
evolution | tung FADX is grouped with DELTA12 fatty acid desaturases and hydroxylases rather than conjugases, which is consistent with its desaturase activity | Vernicia fordii |
additional information | incubation of yeast cells expressing FADX with exogenously supplied palmitoleicacid leads to substantial incorporation of thisfatty acid into yeast cells, accounting for approximately79% of total fatty acids. Oleic acid is reduced to only 3% under these growth conditions | Vernicia fordii |
physiological function | FADX converts linoleic acid into alpha-eleostearic acid. in the plant seed oil | Vernicia fordii |