Literature summary for 1.14.19.35 extracted from

Tovuu, A.; Zulfugarov, I.S.; Wu, G.; Kang, I.S.; Kim, C.; Moon, B.Y.; An, G.; Lee, C.H.
Rice mutants deficient in omega-3 fatty acid desaturase (FAD8) fail to acclimate to cold temperatures (2016), Plant Physiol. Biochem., 109, 525-535 .

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Cloned(Commentary)

Cloned (Comment)	Organism
gene OsFAD8, located on chromosome 7, DNA and amino acid sequence determination and analysis	Oryza sativa Japonica Group

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of knockout mutants of gene OsFAD8, fatty acids contents (mol%) in leaves from wild-type rice and osfad8 mutant lines at 4°C and 28°C, overview	Oryza sativa Japonica Group

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast membrane	-	Oryza sativa Japonica Group	31969	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
alpha-linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+	Oryza sativa Japonica Group	-	alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Oryza sativa Japonica Group	Q84NP3	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Oryza sativa Japonica Group	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpha-linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+	-	Oryza sativa Japonica Group	alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O	-	?

Synonyms

Synonyms	Comment	Organism
FAD8	-	Oryza sativa Japonica Group
omega-3 FA desaturase	-	Oryza sativa Japonica Group
omega-3 fatty acid desaturase	-	Oryza sativa Japonica Group
OsFAD8	-	Oryza sativa Japonica Group

Expression

Organism	Comment	Expression
Oryza sativa Japonica Group	in wild-type plants cold-acclimated at 4°C, the transcript levels of OsFAD8 increase significantly, and the expression levels increase in comparison to those of wild-type and of genes OsFAD7 and OsFAD3. OsFAD8 is a cold-inducible gene	up

General Information

General Information	Comment	Organism
malfunction	osfad8 knockout mutant plants exhibit significant alterations in fatty acid unsaturation for all four investigated plastidic lipid classes. During a 5-day acclimation period at 4°C, further changes in fatty acid unsaturation in both wild-type and mutant plants vary according to the type of lipid. The fluidity of the thylakoid membrane are altered significantly by both FAD8 mutation and cold acclimation, suggesting that factors other than FAD8 are involved in C18 fatty acid unsaturation and fluctuations in membrane fluidity. Similarly, significant changes are noted for both the mutant and wild-type samples in terms of their fatty acid compositions as well as activities related to photosystem (PS) I, PSII, and photoprotection. This includes the development of non-photochemical quenching and increased zeaxanthin accumulation. Despite the relatively small changes in fatty acid composition during cold acclimation, cold-inducible FAD8 knock-out mutants display strong differences in photoprotective activities and a further drop in membrane fluidity. The mutants are more sensitive than wild-type to short-term low-temperature stress that results in increased production of reactive oxygen species after 5 days of chilling. The levels of 18:3 are reduced by 20% in the fad8 knockout mutant compared to wild-type, while the levels of 18:2 are increased 2fold. Phenotype, overview	Oryza sativa Japonica Group
additional information	fatty acids contents (mol%) in leaves from wild-type rice and osfad8 mutant lines at 4°C and 28°C, overview. Membrane fluidity of rice plants is altered with altered fatty acid compositions	Oryza sativa Japonica Group

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Release 2023.1 (January 2023)