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Literature summary for 1.14.19.36 extracted from

  • Sakamoto, T.; Los, D.A.; Higashi, S.; Wada, H.; Nishida, I.; Ohmori, M.; Murata, N.
    Cloning of omega 3 desaturase from cyanobacteria and its use in altering the degree of membrane-lipid unsaturation (1994), Plant Mol. Biol., 26, 249-263.
    View publication on PubMed
Search for more literature for 1.14.19.36

Cloned(Commentary)

Cloned (Comment) Organism
the desA gene, which encodes the DELTA12 desaturase of Synechocystis sp. PCC 6803, and the desB gene are introduced into Synechococcus sp. PCC 7942. Whilst the parent cyanobacterium can only desaturate membrane lipids at the DELTA9 position of fatty acids, the resultant transformant is able to desaturate fatty acids of membrane lipids at the DELTA9, DELTA12 and omega3 positions Synechocystis sp.

Protein Variants

Protein Variants Comment Organism
additional information the desB gene in Synechocystis sp. PCC 6803 is mutated by insertion of a kanamycin-resistance gene cartridge. The resultant mutant is unable to desaturate fatty acids at the omega3 position Synechocystis sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41900
-
 x * 41900, SDS-PAGE Synechocystis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a 1-gamma-linolenoyl 2-acyl-sn-glycerol 3-phosphate + 2 reduced ferredoxin + O2 + 2 H+ Synechocystis sp.
-
 a 1-stearidonoyl 2-acyl-sn-glycerol 3-phosphate + 2 oxidized ferredoxin + 2 H2O
-
 ?
a 1-linoleoyl 2-acyl-sn-glycerol 3-phosphate + 2 reduced ferredoxin + O2 + 2 H+ Synechocystis sp.
-
 a 1-alpha-linolenoyl 2-acyl-sn-glycerol 3-phosphate + 2 oxidized ferredoxin + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Synechocystis sp. Q55240
-
-

Reaction

Reaction Comment Organism Reaction ID
a 1-gamma-linolenoyl 2-acyl-sn-glycerol 3-phosphate + 2 reduced ferredoxin + O2 + 2 H+ = a 1-stearidonoyl 2-acyl-sn-glycerol 3-phosphate + 2 oxidized ferredoxin + 2 H2O (1) Synechocystis sp.
a
a 1-linoleoyl 2-acyl-sn-glycerol 3-phosphate + 2 reduced ferredoxin + O2 + 2 H+ = a 1-alpha-linolenoyl 2-acyl-sn-glycerol 3-phosphate + 2 oxidized ferredoxin + 2 H2O (2) Synechocystis sp.
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a 1-gamma-linolenoyl 2-acyl-sn-glycerol 3-phosphate + 2 reduced ferredoxin + O2 + 2 H+
-
 Synechocystis sp. a 1-stearidonoyl 2-acyl-sn-glycerol 3-phosphate + 2 oxidized ferredoxin + 2 H2O
-
 ?
a 1-linoleoyl 2-acyl-sn-glycerol 3-phosphate + 2 reduced ferredoxin + O2 + 2 H+
-
 Synechocystis sp. a 1-alpha-linolenoyl 2-acyl-sn-glycerol 3-phosphate + 2 oxidized ferredoxin + 2 H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 41900, SDS-PAGE Synechocystis sp.

Synonyms

Synonyms Comment Organism
DesB
-
 Synechocystis sp.

Expression

Organism Comment Expression
Synechocystis sp. the level of the desB transcript in cells grown at 22°C is 10 times higher than that in cells grown at 34°C up