BRENDA - Enzyme Database show
show all sequences of 1.14.19.51

Molecular cloning and characterization of CYP80G2, a cytochrome P450 that catalyzes an intramolecular C-C phenol coupling of (S)-reticuline in magnoflorine biosynthesis, from cultured Coptis japonica cells

Ikezawa, N.; Iwasa, K.; Sato, F.; J. Biol. Chem. 283, 8810-8821 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Saccharomyces cerevisiae
Coptis japonica
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
sequence contains a putative endoplasmic reticulum-localizing signal
Coptis japonica
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-reticuline + [reduced NADPH-hemoprotein reductase] + O2
Coptis japonica
-
(S)-corytuberine + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Coptis japonica
A8CDR5
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Coptis japonica
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R,S)-codamine + [reduced NADPH-hemoprotein reductase] + O2
-
687805
Coptis japonica
2,11-dimethoxy-6-methyl-5,6,6a,7-tetrahydro-4H-dibenzo[de,g]quinoline-1,10-diol + [oxidized NADPH-hemoprotein reductase] + 2 H2O
12% of the activity with reticuline. The major product is (R,S)-orientaline, 4'-O-demethylation product of (R,S)-codamine, and the minor one is proposed to be the same compound as (R,S)-orientaline-derived phenol coupling product
-
-
?
(R,S)-norreticuline + [reduced NADPH-hemoprotein reductase] + O2
-
687805
Coptis japonica
2,10-dimethoxy-5,6,6a,7-tetrahydro-4H-dibenzo[de,g]quinoline-1,11-diol + [oxidized NADPH-hemoprotein reductase] + 2 H2O
19% of the activity with reticuline
-
-
?
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2
-
687805
Coptis japonica
2,11-dimethoxy-6-methyl-5,6,6a,7-tetrahydro-4H-dibenzo[de,g]quinoline-1,10-diol + [oxidized NADPH-hemoprotein reductase] + 2 H2O
2% of the activity with reticuline
-
-
?
(S)-orientaline + [reduced NADPH-hemoprotein reductase] + O2
-
687805
Coptis japonica
2,11-dimethoxy-6-methyl-5,6,6a,7-tetrahydro-4H-dibenzo[de,g]quinoline-1,10-diol + [oxidized NADPH-hemoprotein reductase] + 2 H2O
2.7% of the activity with reticuline
-
-
?
(S)-reticuline + [reduced NADPH-hemoprotein reductase] + O2
-
687805
Coptis japonica
(S)-corytuberine + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
additional information
CC phenol-coupling reaction, requires NADPH and oxygen. The enzyme is specific for S-enantiomer of substrate
687805
Coptis japonica
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Coptis japonica
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Saccharomyces cerevisiae
Coptis japonica
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Coptis japonica
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
sequence contains a putative endoplasmic reticulum-localizing signal
Coptis japonica
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-reticuline + [reduced NADPH-hemoprotein reductase] + O2
Coptis japonica
-
(S)-corytuberine + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Coptis japonica
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R,S)-codamine + [reduced NADPH-hemoprotein reductase] + O2
-
687805
Coptis japonica
2,11-dimethoxy-6-methyl-5,6,6a,7-tetrahydro-4H-dibenzo[de,g]quinoline-1,10-diol + [oxidized NADPH-hemoprotein reductase] + 2 H2O
12% of the activity with reticuline. The major product is (R,S)-orientaline, 4'-O-demethylation product of (R,S)-codamine, and the minor one is proposed to be the same compound as (R,S)-orientaline-derived phenol coupling product
-
-
?
(R,S)-norreticuline + [reduced NADPH-hemoprotein reductase] + O2
-
687805
Coptis japonica
2,10-dimethoxy-5,6,6a,7-tetrahydro-4H-dibenzo[de,g]quinoline-1,11-diol + [oxidized NADPH-hemoprotein reductase] + 2 H2O
19% of the activity with reticuline
-
-
?
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2
-
687805
Coptis japonica
2,11-dimethoxy-6-methyl-5,6,6a,7-tetrahydro-4H-dibenzo[de,g]quinoline-1,10-diol + [oxidized NADPH-hemoprotein reductase] + 2 H2O
2% of the activity with reticuline
-
-
?
(S)-orientaline + [reduced NADPH-hemoprotein reductase] + O2
-
687805
Coptis japonica
2,11-dimethoxy-6-methyl-5,6,6a,7-tetrahydro-4H-dibenzo[de,g]quinoline-1,10-diol + [oxidized NADPH-hemoprotein reductase] + 2 H2O
2.7% of the activity with reticuline
-
-
?
(S)-reticuline + [reduced NADPH-hemoprotein reductase] + O2
-
687805
Coptis japonica
(S)-corytuberine + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
additional information
CC phenol-coupling reaction, requires NADPH and oxygen. The enzyme is specific for S-enantiomer of substrate
687805
Coptis japonica
?
-
-
-
-
Other publictions for EC 1.14.19.51
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
687805
Ikezawa
Molecular cloning and characte ...
Coptis japonica
J. Biol. Chem.
283
8810-8821
2008
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