Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.19.60 extracted from

  • Heemstra, J.R. Jr.; Walsh, C.T.
    Tandem action of the O2- and FADH2-dependent halogenases KtzQ and KtzR produce 6,7-dichlorotryptophan for kutzneride assembly (2008), J. Am. Chem. Soc., 130, 14024-14025.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Kutzneria sp. 744

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.114
-
7-chloro-L-tryptophan pH 7.0, 22°C Kutzneria sp. 744
0.808
-
L-tryptophan pH 7.0, 22°C Kutzneria sp. 744

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7-chloro-L-tryptophan + FADH2 + Cl- + O2 + H+ Kutzneria sp. 744 the enzyme is involved in the biosynthesis of 6,7-dichloro-L-tryptophan, a component of kutznerides (antifungal nonribosomal hexadepsipeptides) 6,7-dichloro-L-tryptophan + FAD + 2 H2O
-
?

Organism

Organism UniProt Comment Textmining
Kutzneria sp. 744 A8CF74
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Kutzneria sp. 744

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-chloro-L-tryptophan + FADH2 + Cl- + O2 + H+
-
Kutzneria sp. 744 6,7-dichloro-L-tryptophan + FAD + 2 H2O
-
?
7-chloro-L-tryptophan + FADH2 + Cl- + O2 + H+ the enzyme is involved in the biosynthesis of 6,7-dichloro-L-tryptophan, a component of kutznerides (antifungal nonribosomal hexadepsipeptides) Kutzneria sp. 744 6,7-dichloro-L-tryptophan + FAD + 2 H2O
-
?
L-tryptophan + FADH2 + Cl- + O2 + H+ kcat/Km for the halogenation of L-tryptophan is less than 1% compared to the value for the halogenation 7-chloro-L-tryptophan Kutzneria sp. 744 6-chloro-L-tryptophan + FAD + 2 H2O
-
?
additional information KtzR is not active for introduction of chloride to piperazic acid or gamma,delta-dehydropiperazic acid Kutzneria sp. 744 ?
-
?

Synonyms

Synonyms Comment Organism
KtzR
-
Kutzneria sp. 744

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at Kutzneria sp. 744

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0013
-
L-tryptophan pH 7.0, 22°C Kutzneria sp. 744
0.023
-
7-chloro-L-tryptophan pH 7.0, 22°C Kutzneria sp. 744

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Kutzneria sp. 744

General Information

General Information Comment Organism
physiological function the enzyme is involved in the biosynthesis of 6,7-dichloro-L-tryptophan, a component of kutznerides (antifungal nonribosomal hexadepsipeptides) Kutzneria sp. 744

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0016
-
L-tryptophan pH 7.0, 22°C Kutzneria sp. 744
0.2
-
7-chloro-L-tryptophan pH 7.0, 22°C Kutzneria sp. 744