BRENDA - Enzyme Database show
show all sequences of 1.14.19.62

Secologanin synthase which catalyzes the oxidative cleavage of loganin into secologanin is a cytochrome P450

Yamamoto, H.; Katano, N.; Ooi, A.; Inoue, K.; Phytochemistry 53, 7-12 (2000)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Ancymidol
complete inhibition
Lonicera japonica
carbon monoxide
inhibition by CO and O2 in the ratio 9 to 1 in the dark, can be reversed by illumination with white light
Lonicera japonica
cytochrome c
partial inhibition
Lonicera japonica
Metyrapone
partial inhibition
Lonicera japonica
miconazole
partial inhibition
Lonicera japonica
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
membrane-associated enzyme
Lonicera japonica
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
loganin + [reduced NADPH-hemoprotein reductase] + O2
Lonicera japonica
-
secologanin + [oxidized NADPH-hemoprotein reductase] + H2O
-
Lonicera japonica
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lonicera japonica
-
-
-
Reaction
Reaction
Commentary
Organism
loganin + [reduced NADPH-hemoprotein reductase] + O2 = secologanin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
enzyme belongs to the group of cytochrome P450 monooxygenases
Lonicera japonica
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
10-days-old cultured cells
Lonicera japonica
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
loganin + [reduced NADPH-hemoprotein reductase] + O2
-
437785
Lonicera japonica
secologanin + [oxidized NADPH-hemoprotein reductase] + H2O
-
437785
Lonicera japonica
?
loganin + [reduced NADPH-hemoprotein reductase] + O2
requires molecular oxygen for oxidative cleavage of the carbon-carbon bond
437785
Lonicera japonica
secologanin + [oxidized NADPH-hemoprotein reductase] + H2O
-
437785
Lonicera japonica
?
additional information
no substrates: loganic acid, 10-hydroxyloganin, 7-epiloganin, 7-dehydrologanin, 7-dehydrologanic acid, 8-epiloganin, verbenalin
437785
Lonicera japonica
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
enzyme assay
Lonicera japonica
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Lonicera japonica
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ancymidol
complete inhibition
Lonicera japonica
carbon monoxide
inhibition by CO and O2 in the ratio 9 to 1 in the dark, can be reversed by illumination with white light
Lonicera japonica
cytochrome c
partial inhibition
Lonicera japonica
Metyrapone
partial inhibition
Lonicera japonica
miconazole
partial inhibition
Lonicera japonica
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
membrane-associated enzyme
Lonicera japonica
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
loganin + [reduced NADPH-hemoprotein reductase] + O2
Lonicera japonica
-
secologanin + [oxidized NADPH-hemoprotein reductase] + H2O
-
Lonicera japonica
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
10-days-old cultured cells
Lonicera japonica
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
loganin + [reduced NADPH-hemoprotein reductase] + O2
-
437785
Lonicera japonica
secologanin + [oxidized NADPH-hemoprotein reductase] + H2O
-
437785
Lonicera japonica
?
loganin + [reduced NADPH-hemoprotein reductase] + O2
requires molecular oxygen for oxidative cleavage of the carbon-carbon bond
437785
Lonicera japonica
secologanin + [oxidized NADPH-hemoprotein reductase] + H2O
-
437785
Lonicera japonica
?
additional information
no substrates: loganic acid, 10-hydroxyloganin, 7-epiloganin, 7-dehydrologanin, 7-dehydrologanic acid, 8-epiloganin, verbenalin
437785
Lonicera japonica
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
enzyme assay
Lonicera japonica
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Lonicera japonica
Other publictions for EC 1.14.19.62
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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4
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4
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1
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1
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1
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4
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1
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3
3
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670558
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localization of tabersonine 16 ...
Catharanthus roseus
Plant J.
44
581-594
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1
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437785
Yamamoto
Secologanin synthase which cat ...
Lonicera japonica
Phytochemistry
53
7-12
2000
-
-
-
-
-
-
5
-
1
-
-
1
-
2
-
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1
-
2
-
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3
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1
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1
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5
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1
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1
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2
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3
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1
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1
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437786
Irmler
Indole alkaloid biosynthesis i ...
Catharanthus roseus
Plant J.
24
797-804
2000
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1
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1
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4
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1
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1
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3
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1
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1
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1
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3
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-
-
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-
437784
Yamamoto
-
Transformation of loganin and ...
Lonicera japonica
Phytochemistry
50
417-422
1999
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-
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1
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1
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1
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1
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1
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