BRENDA - Enzyme Database show
show all sequences of 1.14.19.62

The subcellular organization of strictosidine biosynthesis in Catharanthus roseus epidermis highlights several trans-tonoplast translocations of intermediate metabolites

Guirimand, G.; Guihur, A.; Ginis, O.; Poutrain, P.; Hericourt, F.; Oudin, A.; Lanoue, A.; St-Pierre, B.; Burlat, V.; Courdavault, V.; FEBS J. 278, 749-763 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression of SLS-GFP fusion protein in Catharanthus roseus cells show localization to the endoplasmic reticulum membranes
Catharanthus roseus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
the enzyme is anchored to the endoplasmic reticulum via a N-teminal helix, thus allowing the production of secologanin on the cytosolic side of the endoplasmic reticulum membrane
Catharanthus roseus
5783
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
loganin + [reduced NADPH-hemoprotein reductase] + O2
Catharanthus roseus
-
secologanin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Catharanthus roseus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
epidermis
epidermis-specific expression of SLS
Catharanthus roseus
-
leaf
-
Catharanthus roseus
-
additional information
RT-PCR tissue expression analysis, overview
Catharanthus roseus
-
seedling
-
Catharanthus roseus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
loganin + [reduced NADPH-hemoprotein reductase] + O2
-
724967
Catharanthus roseus
secologanin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
CYP71A1
Catharanthus roseus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of SLS-GFP fusion protein in Catharanthus roseus cells show localization to the endoplasmic reticulum membranes
Catharanthus roseus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
CYP71A1
Catharanthus roseus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
the enzyme is anchored to the endoplasmic reticulum via a N-teminal helix, thus allowing the production of secologanin on the cytosolic side of the endoplasmic reticulum membrane
Catharanthus roseus
5783
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
loganin + [reduced NADPH-hemoprotein reductase] + O2
Catharanthus roseus
-
secologanin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
epidermis
epidermis-specific expression of SLS
Catharanthus roseus
-
leaf
-
Catharanthus roseus
-
additional information
RT-PCR tissue expression analysis, overview
Catharanthus roseus
-
seedling
-
Catharanthus roseus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
loganin + [reduced NADPH-hemoprotein reductase] + O2
-
724967
Catharanthus roseus
secologanin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
General Information
General Information
Commentary
Organism
malfunction
mutant DELTAthSLS fusion protein forms punctuated aggregates in the cytosol in close vicinity with plastids
Catharanthus roseus
metabolism
the enzyme catalyzes the last step of the monoterpene secoiridoid pathway
Catharanthus roseus
additional information
the enzyme is anchored to the endoplasmic reticulum via a N-teminal helix, thus allowing the production of secologanin on the cytosolic side of the endoplasmic reticulum membrane
Catharanthus roseus
General Information (protein specific)
General Information
Commentary
Organism
malfunction
mutant DELTAthSLS fusion protein forms punctuated aggregates in the cytosol in close vicinity with plastids
Catharanthus roseus
metabolism
the enzyme catalyzes the last step of the monoterpene secoiridoid pathway
Catharanthus roseus
additional information
the enzyme is anchored to the endoplasmic reticulum via a N-teminal helix, thus allowing the production of secologanin on the cytosolic side of the endoplasmic reticulum membrane
Catharanthus roseus
Other publictions for EC 1.14.19.62
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744599
Duge de Bernonville
Characterization of a second ...
Catharanthus roseus
BMC Genomics
16
619
2015
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1
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3
3
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724967
Guirimand
The subcellular organization o ...
Catharanthus roseus
FEBS J.
278
749-763
2011
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1
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1
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1
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4
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4
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1
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1
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1
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1
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4
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1
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3
3
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670558
Murata
localization of tabersonine 16 ...
Catharanthus roseus
Plant J.
44
581-594
2005
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1
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3
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437785
Yamamoto
Secologanin synthase which cat ...
Lonicera japonica
Phytochemistry
53
7-12
2000
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5
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1
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1
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5
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3
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1
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437786
Irmler
Indole alkaloid biosynthesis i ...
Catharanthus roseus
Plant J.
24
797-804
2000
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1
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1
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4
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1
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3
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437784
Yamamoto
-
Transformation of loganin and ...
Lonicera japonica
Phytochemistry
50
417-422
1999
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1
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