BRENDA - Enzyme Database show
show all sequences of 1.14.19.62

Characterization of a second secologanin synthase isoform producing both secologanin and secoxyloganin allows enhanced de novo assembly of a Catharanthus roseus transcriptome

Duge de Bernonville, T.; Foureau, E.; Parage, C.; Lanoue, A.; Clastre, M.; Londono, M.A.; Oudin, A.; Houille, B.; Papon, N.; Besseau, S.; Glevarec, G.; Atehortua, L.; Giglioli-Guivarch, N.; St-Pierre, B.; De Luca, V.; O'Connor, S.E.; Courdavault, V.; BMC Genomics 16, 619 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene CYP72C, DNA and amino acid sequence determination and analysis, sequence comparisons, transcriptome analysis, real-time PCR expression analysis
Catharanthus roseus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
an endoplasmic reticulum-anchored enzyme
Catharanthus roseus
5783
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
loganin + [reduced NADPH-hemoprotein reductase] + O2
Catharanthus roseus
-
secologanin + [oxidized NADPH-hemoprotein reductase]+ 2 H2O
-
-
?
additional information
Catharanthus roseus
the second secologanin synthase isoform SLS2 produces both secologanin and secoxyloganin
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Catharanthus roseus
U5NDT8
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
SLS2 might be the prominent isoform of secologanin biosynthesis in the aerial parts of the plant
Catharanthus roseus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
loganin + [reduced NADPH-hemoprotein reductase] + O2
-
744599
Catharanthus roseus
secologanin + [oxidized NADPH-hemoprotein reductase]+ 2 H2O
-
-
-
?
additional information
the second secologanin synthase isoform SLS2 produces both secologanin and secoxyloganin
744599
Catharanthus roseus
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Catharanthus roseus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Catharanthus roseus
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Catharanthus roseus
NADPH-hemoprotein reductase
-
Catharanthus roseus
Cloned(Commentary) (protein specific)
Commentary
Organism
gene CYP72C, DNA and amino acid sequence determination and analysis, sequence comparisons, transcriptome analysis, real-time PCR expression analysis
Catharanthus roseus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Catharanthus roseus
NADPH-hemoprotein reductase
-
Catharanthus roseus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
an endoplasmic reticulum-anchored enzyme
Catharanthus roseus
5783
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
loganin + [reduced NADPH-hemoprotein reductase] + O2
Catharanthus roseus
-
secologanin + [oxidized NADPH-hemoprotein reductase]+ 2 H2O
-
-
?
additional information
Catharanthus roseus
the second secologanin synthase isoform SLS2 produces both secologanin and secoxyloganin
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
SLS2 might be the prominent isoform of secologanin biosynthesis in the aerial parts of the plant
Catharanthus roseus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
loganin + [reduced NADPH-hemoprotein reductase] + O2
-
744599
Catharanthus roseus
secologanin + [oxidized NADPH-hemoprotein reductase]+ 2 H2O
-
-
-
?
additional information
the second secologanin synthase isoform SLS2 produces both secologanin and secoxyloganin
744599
Catharanthus roseus
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Catharanthus roseus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Catharanthus roseus
General Information
General Information
Commentary
Organism
metabolism
the enzyme is important in the monoterpenoid indole alkaloid (MIA) biosynthesis in Catharanthus roseus, subcellular organization of the central steps of the pathway, overview. The ring-opening reaction of loganin in the biosynthesis of secologanin is catalyzed by the fourth P450 of this pathway, secologanin synthase (SLS, CYP72A1). Both isozymes SLS1 and SLS2 not only catalyze the oxidative ring cleavage of loganin to produce secologanin but also perform the oxidation of secologanin into secoxyloganin. SLS1 and SLS2 constitute the third type of P450 from the seco-iridoid pathway performing more than one catalytic reaction. Additional enzymes might convert secoxyloganin back to secologanin, the subcellular compartmentation of secologanin biosynthesis may also limit secoxyloganin formation in planta. Secoxyloganin is an acidic compound derived from secologanin that is no longer able to be condensed with tryptamine by STR in the vacuole, due to the absence of the aldehyde function. If secoxyloganin formation occurs in vivo, the resulting depletion of the secologanin pool is deleterious for the subsequent synthesis of MIAs
Catharanthus roseus
additional information
consensus transcriptome analysis of Catharanthus roseus, detailed overview
Catharanthus roseus
physiological function
secologanin synthases (SLS) are P450 enzymes that catalyze an unusual ring-opening reaction of loganin in the biosynthesis of the monoterpenoid indole alkaloids (MIA) precursor secologanin
Catharanthus roseus
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is important in the monoterpenoid indole alkaloid (MIA) biosynthesis in Catharanthus roseus, subcellular organization of the central steps of the pathway, overview. The ring-opening reaction of loganin in the biosynthesis of secologanin is catalyzed by the fourth P450 of this pathway, secologanin synthase (SLS, CYP72A1). Both isozymes SLS1 and SLS2 not only catalyze the oxidative ring cleavage of loganin to produce secologanin but also perform the oxidation of secologanin into secoxyloganin. SLS1 and SLS2 constitute the third type of P450 from the seco-iridoid pathway performing more than one catalytic reaction. Additional enzymes might convert secoxyloganin back to secologanin, the subcellular compartmentation of secologanin biosynthesis may also limit secoxyloganin formation in planta. Secoxyloganin is an acidic compound derived from secologanin that is no longer able to be condensed with tryptamine by STR in the vacuole, due to the absence of the aldehyde function. If secoxyloganin formation occurs in vivo, the resulting depletion of the secologanin pool is deleterious for the subsequent synthesis of MIAs
Catharanthus roseus
additional information
consensus transcriptome analysis of Catharanthus roseus, detailed overview
Catharanthus roseus
physiological function
secologanin synthases (SLS) are P450 enzymes that catalyze an unusual ring-opening reaction of loganin in the biosynthesis of the monoterpenoid indole alkaloids (MIA) precursor secologanin
Catharanthus roseus
Other publictions for EC 1.14.19.62
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744599
Duge de Bernonville
Characterization of a second ...
Catharanthus roseus
BMC Genomics
16
619
2015
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724967
Guirimand
The subcellular organization o ...
Catharanthus roseus
FEBS J.
278
749-763
2011
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670558
Murata
localization of tabersonine 16 ...
Catharanthus roseus
Plant J.
44
581-594
2005
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437785
Yamamoto
Secologanin synthase which cat ...
Lonicera japonica
Phytochemistry
53
7-12
2000
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437786
Irmler
Indole alkaloid biosynthesis i ...
Catharanthus roseus
Plant J.
24
797-804
2000
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437784
Yamamoto
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Transformation of loganin and ...
Lonicera japonica
Phytochemistry
50
417-422
1999
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