BRENDA - Enzyme Database show
show all sequences of 1.14.19.64

Characterization of two methylenedioxy bridge-forming cytochrome P450-dependent enzymes of alkaloid formation in the Mexican prickly poppy Argemone mexicana

Diaz Chavez, M.L.; Rolf, M.; Gesell, A.; Kutchan, T.M.; Arch. Biochem. Biophys. 507, 186-193 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Spodoptera frugiperda Sf9 cells
Argemone mexicana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.2
-
(S)-cheilanthifoline
pH 8.0, 30C, kcat: 100%
Argemone mexicana
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
57450
-
calculated from cDNA
Argemone mexicana
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Argemone mexicana
B1NF19
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Argemone mexicana
-
plantlet
-
Argemone mexicana
-
root
highly expressed
Argemone mexicana
-
stem
-
Argemone mexicana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-cheilanthifoline + [reduced NADPH-hemoprotein reductase] + O2
-
711025
Argemone mexicana
(S)-stylopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(S)-coreximine + [reduced NADPH-hemoprotein reductase] + O2
kcat (S)-coreximine: 1% compared to kcat (S)-cheilanthifoline: 100%
711025
Argemone mexicana
(S)-cheilanthifoline + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Argemone mexicana
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
30
35
-
Argemone mexicana
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kcat (S)-coreximine: 1% compared to kcat (S)-cheilanthifoline: 100%
Argemone mexicana
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at, optimal pH using S-cheilanthifoline as a substrate
Argemone mexicana
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
10
half optimal activity at pH 6.5 and pH 9 using S-cheilanthifoline as a substrate
Argemone mexicana
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Spodoptera frugiperda Sf9 cells
Argemone mexicana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.2
-
(S)-cheilanthifoline
pH 8.0, 30C, kcat: 100%
Argemone mexicana
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
57450
-
calculated from cDNA
Argemone mexicana
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Argemone mexicana
-
plantlet
-
Argemone mexicana
-
root
highly expressed
Argemone mexicana
-
stem
-
Argemone mexicana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-cheilanthifoline + [reduced NADPH-hemoprotein reductase] + O2
-
711025
Argemone mexicana
(S)-stylopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(S)-coreximine + [reduced NADPH-hemoprotein reductase] + O2
kcat (S)-coreximine: 1% compared to kcat (S)-cheilanthifoline: 100%
711025
Argemone mexicana
(S)-cheilanthifoline + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Argemone mexicana
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
30
35
-
Argemone mexicana
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kcat (S)-coreximine: 1% compared to kcat (S)-cheilanthifoline: 100%
Argemone mexicana
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at, optimal pH using S-cheilanthifoline as a substrate
Argemone mexicana
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
10
half optimal activity at pH 6.5 and pH 9 using S-cheilanthifoline as a substrate
Argemone mexicana
General Information
General Information
Commentary
Organism
physiological function
CYP719A13 can be involved in both sanguinarine and berberine formation in Argemone mexicana
Argemone mexicana
General Information (protein specific)
General Information
Commentary
Organism
physiological function
CYP719A13 can be involved in both sanguinarine and berberine formation in Argemone mexicana
Argemone mexicana
Other publictions for EC 1.14.19.64
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746047
Yahyazadeh
-
Cloning and characterization ...
Chelidonium majus
Plant Cell Physiol.
58
1421-1430
2017
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
746447
Hori
Efficient microbial productio ...
Eschscholzia californica
Sci. Rep.
6
22201
2016
-
2
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
728446
Takemura
Molecular cloning and characte ...
Eschscholzia californica
Phytochemistry
91
100-108
2013
-
-
-
-
-
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
711025
Diaz Chavez
Characterization of two methyl ...
Argemone mexicana
Arch. Biochem. Biophys.
507
186-193
2011
-
-
1
-
-
-
-
1
-
-
1
-
-
5
-
-
-
-
-
4
-
-
2
-
1
1
-
1
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
4
-
-
2
-
1
1
-
1
1
1
-
-
-
1
1
-
-
-
673579
Ikezawa
Molecular cloning and characte ...
Eschscholzia californica
FEBS J.
274
1019-1035
2007
-
-
1
-
-
-
-
2
-
-
-
3
-
7
-
-
-
-
-
3
2
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
3
-
-
-
-
-
3
2
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
395597
Bauer
-
Two methylenedioxy bridge form ...
Eschscholzia californica
Phytochemistry
30
2953-2961
1991
-
-
-
-
-
-
-
1
1
-
-
1
-
1
-
-
-
-
-
1
-
1
2
-
1
-
2
-
1
1
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
1
-
1
2
-
1
-
2
-
1
1
-
-
-
-
-
-
-
-