Cloned (Comment) | Organism |
---|---|
expressed in Spodoptera frugiperda Sf9 cells | Argemone mexicana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
(S)-scoulerine | pH 8.0, 30°C, kcat: 100% | Argemone mexicana | |
8.1 | - |
(S)-scoulerine | pH 8.0, 30°C, kcat: 29% | Argemone mexicana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57450 | - |
calculated from cDNA | Argemone mexicana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Argemone mexicana | B1NF19 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Argemone mexicana | - |
plantlet | - |
Argemone mexicana | - |
root | highly expressed | Argemone mexicana | - |
stem | - |
Argemone mexicana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-coreximine + [reduced NADPH-hemoprotein reductase] + O2 | kcat (S)-coreximine: 1% compared to kcat (S)-cheilanthifoline: 100% | Argemone mexicana | (S)-cheilanthifoline + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
(S)-scoulerine + [reduced NADPH-hemoprotein reductase] + O2 | - |
Argemone mexicana | (S)-cheilanthifoline + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
(S)-scoulerine + [reduced NADPH-hemoprotein reductase] + O2 | - |
Argemone mexicana | (S)-nandinine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP719A13 | - |
Argemone mexicana |
CYP719A14 | - |
Argemone mexicana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Argemone mexicana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 35 | - |
Argemone mexicana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kcat (S)-coreximine: 1% compared to kcat (S)-cheilanthifoline: 100% | Argemone mexicana | |
additional information | - |
additional information | kcat (S)-scoulerine: 100%, pH8, 30°C | Argemone mexicana | |
additional information | - |
additional information | kcat (S)-scoulerine: 29%, kcat (S)-cheilanthifoline: 100% | Argemone mexicana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
optimal pH | Argemone mexicana |
8 | - |
assay at | Argemone mexicana |
8 | - |
assay at, optimal pH using S-cheilanthifoline as a substrate | Argemone mexicana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | half optimal activity at pH 6.5 and 8.5 | Argemone mexicana |
6 | 10 | half optimal activity at pH 6.5 and pH 9 using S-cheilanthifoline as a substrate | Argemone mexicana |
General Information | Comment | Organism |
---|---|---|
physiological function | CYP719A13 can be involved in both sanguinarine and berberine formation in Argemone mexicana | Argemone mexicana |