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Literature summary for 1.14.19.66 extracted from
- Molecular cloning and heterologous expression of a cDNA encoding berbamunine synthase, a C-O phenol-coupling cytochrome P450 from the higher plant Berberis stolonifera (1995), Proc. Natl. Acad. Sci. USA, 92, 2071-2075.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in functional form in insect cell culture using a baculovirus-based expression system | Berberis stolonifera |
| expression in Sf9 cells | Berberis stolonifera |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | normal Michaelis-Menten kinetics are not observed for the formation of guattegaumerine or berbamunine | Berberis stolonifera |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
x * 46000, SDS-PAGE | Berberis stolonifera |
| 55474 | - |
x * 55474, calculation from nucleotide sequence | Berberis stolonifera |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Berberis stolonifera | P47195 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| heterologously expressed enzyme from Sf9 cell culture | Berberis stolonifera |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Berberis stolonifera | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 (S)-N-methylcoclaurine + 2 (R)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2 | - |
Berberis stolonifera | berbamunine + guattegaumerine + [oxidized NADPH-hemoprotein reductase] + H2O | two molecules of (R)-N-methylcoclaurine for the (R,R) dimer guattegaumerine or one molecule each of (R)-N-methylcoclaurine and (S)-N-methylcoclaurine form the (R,S) dimer berbamunine. The ratio of the two bisbenzylisoquinolines formed can be altered by the reductase source or by varying the enantiomer composition of the substrates | ? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 46000, SDS-PAGE | Berberis stolonifera |
| ? | x * 55474, calculation from nucleotide sequence | Berberis stolonifera |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
formation of berbamunine | Berberis stolonifera |
| 38 | - |
formation of guattegaumerine | Berberis stolonifera |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 7 | 49 | 50% of maximal activity at 7°C and 49°C, formation of berbamunine | Berberis stolonifera |
| 18 | 46 | 50% of maximal activity at 18°C and 46°C, formation of guattegaumerine | Berberis stolonifera |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Berberis stolonifera |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
formation of berbamunine or guattegaumerine | Berberis stolonifera |
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