BRENDA - Enzyme Database show
show all sequences of 1.14.19.66

Heterologous expression of alkaloid biosynthetic genes - a review

Kutchan, T.M.; Gene 179, 73-81 (1996)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Berberis stolonifera
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Berberis stolonifera
P47195
-
-
Purification (Commentary)
Commentary
Organism
-
Berberis stolonifera
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
additional information
-
-
Berberis stolonifera
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-N-methylcoclaurine + (R)-N-methylcoclaurine + (S)-coclaurine + [reduced NADPH-hemoprotein reductase] + O2
-
393214
Berberis stolonifera
(R,S)-berbamunine + (R,R)-guattegaumerine + (R,S)-2'-norberbamunine + [oxidized NADPH-hemoprotein reductase] + H2O
-
393214
Berberis stolonifera
-
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Berberis stolonifera
Purification (Commentary) (protein specific)
Commentary
Organism
-
Berberis stolonifera
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
additional information
-
-
Berberis stolonifera
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-N-methylcoclaurine + (R)-N-methylcoclaurine + (S)-coclaurine + [reduced NADPH-hemoprotein reductase] + O2
-
393214
Berberis stolonifera
(R,S)-berbamunine + (R,R)-guattegaumerine + (R,S)-2'-norberbamunine + [oxidized NADPH-hemoprotein reductase] + H2O
-
393214
Berberis stolonifera
-
Other publictions for EC 1.14.19.66
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
393214
Kutchan
Heterologous expression of alk ...
Berberis stolonifera
Gene
179
73-81
1996
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1
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4
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1
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1
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1
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1
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1
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1
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1
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393213
Kraus
Molecular cloning and heterolo ...
Berberis stolonifera
Proc. Natl. Acad. Sci. USA
92
2071-2075
1995
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-
1
-
-
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1
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2
-
-
3
-
-
1
-
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3
-
-
1
1
2
2
-
1
1
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-
-
-
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1
-
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-
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1
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2
-
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1
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3
-
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1
1
2
2
-
1
1
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393212
Stadler
The purification and character ...
Berberis stolonifera
J. Biol. Chem.
268
823-831
1993
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1
1
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1
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1
1
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1
1
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4
1
1
1
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1
1
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1
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1
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1
1
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1
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1
1
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4
1
1
1
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1
1
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