BRENDA - Enzyme Database show
show all sequences of 1.14.19.67

Purification and characterization of a cytochrome p450 enzyme from pig liver, catalyzing the phenol oxidative coupling of (R)-reticuline to salutaridine, the critical step in morphine biosynthesis

Amann, T.; Roos, P.H.; Huh, H.; Zenk, M.H.; Heterocycles 1, 425-440 (1995)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.003
-
(R)-reticuline
-
Sus scrofa
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
lysosome
-
Sus scrofa
5764
-
microsome
-
Bos taurus
-
-
microsome
-
Homo sapiens
-
-
microsome
-
Mus musculus
-
-
microsome
-
Ovis aries
-
-
microsome
-
Rattus norvegicus
-
-
microsome
-
Sus scrofa
-
-
mitochondrion
-
Mus musculus
5739
-
mitochondrion
light mitochondrial fraction
Sus scrofa
5739
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
x * 50000, SDS-PAGE
Sus scrofa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-reticuline + [reduced NADPH-hemoprotein reductase] + O2
Sus scrofa
critical step in morphine biosynthesis
salutaridine + [oxidized NADPH-hemoprotein reductase] + H2O
-
Sus scrofa
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
-
-
Homo sapiens
-
very low activity
-
Mus musculus
-
-
-
Ovis aries
-
-
-
Rattus norvegicus
-
-
-
Sus scrofa
-
-
-
Purification (Commentary)
Commentary
Organism
-
Sus scrofa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Sus scrofa
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-reticuline + [reduced NADPH-hemoprotein reductase] + O2
highly regioselective and stereoselective enzyme
393876
Sus scrofa
salutaridine + [oxidized NADPH-hemoprotein reductase] + H2O
-
393876
Sus scrofa
-
(R)-reticuline + [reduced NADPH-hemoprotein reductase] + O2
critical step in morphine biosynthesis
393876
Sus scrofa
salutaridine + [oxidized NADPH-hemoprotein reductase] + H2O
-
393876
Sus scrofa
-
(R/S)-orientaline + [reduced NADPH-hemoprotein reductase] + O2
-
393876
Sus scrofa
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 50000, SDS-PAGE
Sus scrofa
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Bos taurus
cytochrome P450
-
Homo sapiens
cytochrome P450
-
Mus musculus
cytochrome P450
-
Ovis aries
cytochrome P450
-
Rattus norvegicus
cytochrome P450
20 nmol cytochrome P450 per mg of enzyme; enzyme contains cytochrome P450
Sus scrofa
N5-Dinitrophenyl-D-ornithine methyl ester
-
Bos taurus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Bos taurus
cytochrome P450
-
Homo sapiens
cytochrome P450
-
Mus musculus
cytochrome P450
-
Ovis aries
cytochrome P450
-
Rattus norvegicus
cytochrome P450
20 nmol cytochrome P450 per mg of enzyme; enzyme contains cytochrome P450
Sus scrofa
N5-Dinitrophenyl-D-ornithine methyl ester
-
Bos taurus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.003
-
(R)-reticuline
-
Sus scrofa
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
lysosome
-
Sus scrofa
5764
-
microsome
-
Bos taurus
-
-
microsome
-
Homo sapiens
-
-
microsome
-
Mus musculus
-
-
microsome
-
Ovis aries
-
-
microsome
-
Rattus norvegicus
-
-
microsome
-
Sus scrofa
-
-
mitochondrion
-
Mus musculus
5739
-
mitochondrion
light mitochondrial fraction
Sus scrofa
5739
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
x * 50000, SDS-PAGE
Sus scrofa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-reticuline + [reduced NADPH-hemoprotein reductase] + O2
Sus scrofa
critical step in morphine biosynthesis
salutaridine + [oxidized NADPH-hemoprotein reductase] + H2O
-
Sus scrofa
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Sus scrofa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Sus scrofa
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-reticuline + [reduced NADPH-hemoprotein reductase] + O2
highly regioselective and stereoselective enzyme
393876
Sus scrofa
salutaridine + [oxidized NADPH-hemoprotein reductase] + H2O
-
393876
Sus scrofa
-
(R)-reticuline + [reduced NADPH-hemoprotein reductase] + O2
critical step in morphine biosynthesis
393876
Sus scrofa
salutaridine + [oxidized NADPH-hemoprotein reductase] + H2O
-
393876
Sus scrofa
-
(R/S)-orientaline + [reduced NADPH-hemoprotein reductase] + O2
-
393876
Sus scrofa
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 50000, SDS-PAGE
Sus scrofa
Other publictions for EC 1.14.19.67
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743574
Fossati
Synthesis of morphinan alkalo ...
Papaver somniferum
PLoS ONE
10
e0124459
2015
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
728475
Onoyovwe
Morphine biosynthesis in opium ...
Papaver somniferum
Plant Cell
25
4110-4122
2013
-
-
-
-
-
-
-
-
-
-
1
1
-
2
-
-
-
-
-
5
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
5
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
720707
Wijekoon
Systematic knockdown of morphi ...
Papaver somniferum
Plant J.
69
1052-1063
2012
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
698930
Gesell
CYP719B1 is salutaridine synth ...
Papaver somniferum
J. Biol. Chem.
284
24432-24442
2009
-
-
1
-
-
-
-
1
-
-
1
1
-
4
-
-
1
-
-
-
-
-
2
-
2
-
-
1
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
-
1
1
-
-
-
1
-
-
-
-
2
-
2
-
-
1
2
-
-
-
-
-
-
-
-
-
393876
Amann
-
Purification and characterizat ...
Bos taurus, Homo sapiens, Mus musculus, Ovis aries, Rattus norvegicus, Sus scrofa
Heterocycles
1
425-440
1995
-
-
-
-
-
-
-
1
9
-
1
1
-
6
-
-
1
-
-
1
-
-
3
1
-
-
-
-
-
-
-
7
-
-
-
-
-
-
7
-
-
-
-
-
-
1
9
-
1
1
-
-
-
1
-
1
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
393875
Gerardy R.; Zenk
-
Formation of salutaridine from ...
Papaver somniferum
Phytochemistry
32
79-86
1993
-
-
-
-
-
-
3
2
2
-
-
1
-
1
-
-
-
1
-
5
-
4
2
-
1
1
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
3
-
2
2
-
-
1
-
-
-
-
-
5
-
4
2
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-