BRENDA - Enzyme Database show
show all sequences of 1.14.19.68

Canadine synthase from Thalictrum tuberosum cell cultures catalyses the formation of the methylenedioxy bridge in berberine synthesis

Rueffer, M.; Zenk, M.H.; Phytochemistry 36, 1219-1223 (1994)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
cytochrome c
microsomal-bound enzyme
Thalictrum tuberosum
juglone
microsomal-bound enzyme
Thalictrum tuberosum
ketoconazole
microsomal-bound enzyme
Thalictrum tuberosum
menadione
microsomal-bound enzyme
Thalictrum tuberosum
Metyrapone
microsomal-bound enzyme
Thalictrum tuberosum
Plumbagin
microsomal-bound enzyme
Thalictrum tuberosum
Prochloraz
microsomal-bound enzyme
Thalictrum tuberosum
Propiconazole
microsomal-bound enzyme
Thalictrum tuberosum
Tetcyclacis
microsomal-bound enzyme
Thalictrum tuberosum
Triadimefone
microsomal-bound enzyme
Thalictrum tuberosum
tropolone
microsomal-bound enzyme
Thalictrum tuberosum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0115
-
tetrahydrocolumbamine
canadine synthase in complex with a cytochrome P-450 reductase
Thalictrum tuberosum
0.033
-
NADPH
canadine synthase in complex with a cytochrome P-450 reductase
Thalictrum tuberosum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Thalictrum tuberosum
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2
Thalictrum tuberosum
penultimate step in the biosynthesis of the protoberberine alkaloid, berberine
(S)-canadine + [oxidized NADPH-hemoprotein reductase] + H2O
(S)-canadine is identical with (S)-tetrahydroberberine
Thalictrum tuberosum
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Berberis aristata
-
-
-
Berberis crataegina
-
-
-
Berberis henryana
-
-
-
Berberis stolonifera
-
-
-
Berberis taliensis
-
-
-
Coptis japonica
-
-
-
Thalictrum glabrum
-
-
-
Thalictrum glaucum
-
-
-
Thalictrum macrocarpum
-
-
-
Thalictrum tuberosum
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Coptis japonica
-
cell culture
protoberberine producing cell line
Thalictrum tuberosum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2
high substrate specificity
393236
Thalictrum tuberosum
(S)-canadine + [oxidized NADPH-hemoprotein reductase] + H2O
(S)-canadine is identical with (S)-tetrahydroberberine
393236
Thalictrum tuberosum
-
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2
penultimate step in the biosynthesis of the protoberberine alkaloid, berberine
393236
Thalictrum tuberosum
(S)-canadine + [oxidized NADPH-hemoprotein reductase] + H2O
(S)-canadine is identical with (S)-tetrahydroberberine
393236
Thalictrum tuberosum
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
canadine synthase in complex with a cytochrome P-450 reductase
Thalictrum tuberosum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
canadine synthase in complex with a cytochrome P-450 reductase
Thalictrum tuberosum
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
the enzyme forms a complex with a cytochrome P-450 reductase
Thalictrum tuberosum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
the enzyme forms a complex with a cytochrome P-450 reductase
Thalictrum tuberosum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
cytochrome c
microsomal-bound enzyme
Thalictrum tuberosum
juglone
microsomal-bound enzyme
Thalictrum tuberosum
ketoconazole
microsomal-bound enzyme
Thalictrum tuberosum
menadione
microsomal-bound enzyme
Thalictrum tuberosum
Metyrapone
microsomal-bound enzyme
Thalictrum tuberosum
Plumbagin
microsomal-bound enzyme
Thalictrum tuberosum
Prochloraz
microsomal-bound enzyme
Thalictrum tuberosum
Propiconazole
microsomal-bound enzyme
Thalictrum tuberosum
Tetcyclacis
microsomal-bound enzyme
Thalictrum tuberosum
Triadimefone
microsomal-bound enzyme
Thalictrum tuberosum
tropolone
microsomal-bound enzyme
Thalictrum tuberosum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0115
-
tetrahydrocolumbamine
canadine synthase in complex with a cytochrome P-450 reductase
Thalictrum tuberosum
0.033
-
NADPH
canadine synthase in complex with a cytochrome P-450 reductase
Thalictrum tuberosum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Thalictrum tuberosum
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2
Thalictrum tuberosum
penultimate step in the biosynthesis of the protoberberine alkaloid, berberine
(S)-canadine + [oxidized NADPH-hemoprotein reductase] + H2O
(S)-canadine is identical with (S)-tetrahydroberberine
Thalictrum tuberosum
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Coptis japonica
-
cell culture
protoberberine producing cell line
Thalictrum tuberosum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2
high substrate specificity
393236
Thalictrum tuberosum
(S)-canadine + [oxidized NADPH-hemoprotein reductase] + H2O
(S)-canadine is identical with (S)-tetrahydroberberine
393236
Thalictrum tuberosum
-
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2
penultimate step in the biosynthesis of the protoberberine alkaloid, berberine
393236
Thalictrum tuberosum
(S)-canadine + [oxidized NADPH-hemoprotein reductase] + H2O
(S)-canadine is identical with (S)-tetrahydroberberine
393236
Thalictrum tuberosum
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
canadine synthase in complex with a cytochrome P-450 reductase
Thalictrum tuberosum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
canadine synthase in complex with a cytochrome P-450 reductase
Thalictrum tuberosum
Other publictions for EC 1.14.19.68
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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1
1
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1
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744778
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Coptis chinensis
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1
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1
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3
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1
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1
-
1
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1
1
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1
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1
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3
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-
-
1
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1
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1
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727532
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2
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4
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1
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1
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1
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1
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1
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1
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4
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1
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1
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1
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-
1
1
-
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711025
Diaz Chavez
Characterization of two methyl ...
Argemone mexicana
Arch. Biochem. Biophys.
507
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2011
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1
-
-
-
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1
-
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1
-
-
5
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-
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-
-
4
-
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1
-
1
1
-
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1
1
-
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1
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1
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1
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4
-
-
1
-
1
1
-
-
1
1
-
-
-
1
1
-
-
-
676416
Samanani
Cell type-specific localizatio ...
Thalictrum flavum ssp. glaucum
Plant Cell
17
915-926
2005
-
-
-
-
-
-
-
-
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1
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1
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1
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-
393236
Rueffer
-
Canadine synthase from Thalict ...
Berberis aristata, Berberis crataegina, Berberis henryana, Berberis stolonifera, Berberis taliensis, Coptis japonica, Thalictrum glabrum, Thalictrum glaucum, Thalictrum macrocarpum, Thalictrum tuberosum
Phytochemistry
36
1219-1223
1994
-
-
-
-
-
-
11
2
1
-
-
1
-
10
-
-
-
-
-
2
-
-
2
-
1
-
-
-
1
-
-
1
-
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-
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-
-
1
-
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-
-
11
-
2
1
-
-
1
-
-
-
-
-
2
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
393237
Rueffer
-
New reactions in the protoberb ...
Berberidaceae, Ranunculaceae
Chem. Listy
87
215-217
1993
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2
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2
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2
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6
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2
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2
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2
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2
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6
-
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15195
Galneder
Alternative final steps in ber ...
Coptis japonica
Plant Cell Rep.
7
1-4
1988
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1
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1
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1
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1
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