BRENDA - Enzyme Database show
show all sequences of 1.14.19.68

Cloning and characterization of canadine synthase involved in noscapine biosynthesis in opium poppy

Dang, T.T.; Facchini, P.J.; FEBS Lett. 588, 198-204 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Saccharomyces cerevisiae strain YPL 154C:Pep4
Papaver somniferum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00463
-
(S)-tetrahydrocolumbamine
in 100 mM HEPES-NaOH, pH 7.5, at 30°C
Papaver somniferum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2
Papaver somniferum
strict substrate specificity and high affinity for (S)-tetrahydrocolumbamine
(S)-canadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Papaver somniferum
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower bud
-
Papaver somniferum
-
leaf
-
Papaver somniferum
-
root
lowest expression in root
Papaver somniferum
-
stem
highest expression in stem
Papaver somniferum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2
strict substrate specificity and high affinity for (S)-tetrahydrocolumbamine
727532
Papaver somniferum
(S)-canadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Papaver somniferum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Papaver somniferum
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Saccharomyces cerevisiae strain YPL 154C:Pep4
Papaver somniferum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00463
-
(S)-tetrahydrocolumbamine
in 100 mM HEPES-NaOH, pH 7.5, at 30°C
Papaver somniferum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2
Papaver somniferum
strict substrate specificity and high affinity for (S)-tetrahydrocolumbamine
(S)-canadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower bud
-
Papaver somniferum
-
leaf
-
Papaver somniferum
-
root
lowest expression in root
Papaver somniferum
-
stem
highest expression in stem
Papaver somniferum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2
strict substrate specificity and high affinity for (S)-tetrahydrocolumbamine
727532
Papaver somniferum
(S)-canadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Papaver somniferum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Papaver somniferum
General Information
General Information
Commentary
Organism
malfunction
virus-induced gene silencing of CYP719A21 causes a significant increase in (S)-tetrahydrocolumbamine accumulation and a corresponding decrease in the levels of putative downstream intermediates and noscapine
Papaver somniferum
General Information (protein specific)
General Information
Commentary
Organism
malfunction
virus-induced gene silencing of CYP719A21 causes a significant increase in (S)-tetrahydrocolumbamine accumulation and a corresponding decrease in the levels of putative downstream intermediates and noscapine
Papaver somniferum
Other publictions for EC 1.14.19.68
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743234
Galanie
Optimization of yeast-based p ...
Thalictrum flavum subsp. glaucum
Microb. Cell Fact.
14
144
2015
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1
1
-
-
-
-
-
1
-
-
-
-
3
-
-
-
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-
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-
-
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-
-
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1
1
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1
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-
-
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-
744778
He
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Isolation and characterizatio ...
Coptis chinensis
Electron. J. Biotechnol.
18
376-380
2015
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
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1
-
-
3
-
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-
1
-
-
-
-
-
-
-
1
-
1
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-
-
1
1
-
-
-
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-
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-
1
-
-
-
-
1
-
3
-
-
-
1
-
-
-
-
-
-
-
1
-
1
1
-
-
-
727532
Dang
Cloning and characterization o ...
Papaver somniferum
FEBS Lett.
588
198-204
2014
-
-
1
-
-
-
-
1
-
-
-
1
-
2
-
-
-
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-
4
-
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1
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1
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1
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1
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1
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1
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-
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4
-
-
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
711025
Diaz Chavez
Characterization of two methyl ...
Argemone mexicana
Arch. Biochem. Biophys.
507
186-193
2011
-
-
1
-
-
-
-
1
-
-
1
-
-
5
-
-
-
-
-
4
-
-
1
-
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
4
-
-
1
-
1
1
-
-
1
1
-
-
-
1
1
-
-
-
676416
Samanani
Cell type-specific localizatio ...
Thalictrum flavum ssp. glaucum
Plant Cell
17
915-926
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
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1
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1
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-
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-
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-
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-
393236
Rueffer
-
Canadine synthase from Thalict ...
Berberis aristata, Berberis crataegina, Berberis henryana, Berberis stolonifera, Berberis taliensis, Coptis japonica, Thalictrum glabrum, Thalictrum glaucum, Thalictrum macrocarpum, Thalictrum tuberosum
Phytochemistry
36
1219-1223
1994
-
-
-
-
-
-
11
2
1
-
-
1
-
10
-
-
-
-
-
2
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
11
-
2
1
-
-
1
-
-
-
-
-
2
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
393237
Rueffer
-
New reactions in the protoberb ...
Berberidaceae, Ranunculaceae
Chem. Listy
87
215-217
1993
-
-
-
-
-
-
2
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
-
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-
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
15195
Galneder
Alternative final steps in ber ...
Coptis japonica
Plant Cell Rep.
7
1-4
1988
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
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1
-
1
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1
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