BRENDA - Enzyme Database
show all sequences of 1.14.19.73

Molecular cloning and characterization of methylenedioxy bridge-forming enzymes involved in stylopine biosynthesis in Eschscholzia californica

Ikezawa, N.; Iwasa, K.; Sato, F.; FEBS J. 274, 1019-1035 (2007)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Saccharomyces cerevisiae
Eschscholzia californica
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00054
-
(S)-scoulerine
microsomal protein, pH 7.6, 30°C
Eschscholzia californica
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
sequence contains a putative endoplasmic reticulum-localizing signal
Eschscholzia californica
5783
-
microsome
-
Eschscholzia californica
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(R,S)-cheilanthifoline + [reduced NADPH-hemoprotein reductase] + O2
Eschscholzia californica
-
(R,S)-stylopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
(S)-scoulerine + [reduced NADPH-hemoprotein reductase] + O2
Eschscholzia californica
-
(S)-nandinine + [oxidized NADPH-hemoprotein reductase] + H2O
enzyme does not catalyze the methylenedioxy bridge-formation in ring D of scoulerine but strictly recognizes ring A of the substrate
-
?
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2
Eschscholzia californica
-
(S)-tetrahydroberberine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
additional information
Eschscholzia californica
no substrates: columbamine, (R,S)-reticuline, (R,S)-norreticuline, (S)-N-methylcoclaurine, (S)-coclaurine, (R,S)-6-O-methylnorlaudanosoline, and magnoflorine
?
-
-
-
Organism
Organism
UniProt
Commentary
Textmining
Eschscholzia californica
Q50LH4
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Eschscholzia californica
-
root
30fold higher expression than in leaf
Eschscholzia californica
-
stem
-
Eschscholzia californica
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(R,S)-cheilanthifoline + [reduced NADPH-hemoprotein reductase] + O2
-
673579
Eschscholzia californica
(R,S)-stylopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(R,S)-cheilanthifoline + [reduced NADPH-hemoprotein reductase] + O2
-
673579
Eschscholzia californica
stylopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(S)-scoulerine + [reduced NADPH-hemoprotein reductase] + O2
-
673579
Eschscholzia californica
(S)-nandinine + [oxidized NADPH-hemoprotein reductase] + H2O
enzyme does not catalyze the methylenedioxy bridge-formation in ring D of scoulerine but strictly recognizes ring A of the substrate
-
-
?
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2
-
673579
Eschscholzia californica
(S)-tetrahydroberberine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
no substrates: columbamine, (R,S)-reticuline, (R,S)-norreticuline, (S)-N-methylcoclaurine, (S)-coclaurine, (R,S)-6-O-methylnorlaudanosoline, and magnoflorine
673579
Eschscholzia californica
?
-
-
-
-
Synonyms
Synonyms
Commentary
Organism
(S)-stylopine synthase 2
-
Eschscholzia californica
CYP719A3
-
Eschscholzia californica
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Saccharomyces cerevisiae
Eschscholzia californica
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00054
-
(S)-scoulerine
microsomal protein, pH 7.6, 30°C
Eschscholzia californica
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
sequence contains a putative endoplasmic reticulum-localizing signal
Eschscholzia californica
5783
-
microsome
-
Eschscholzia californica
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(R,S)-cheilanthifoline + [reduced NADPH-hemoprotein reductase] + O2
Eschscholzia californica
-
(R,S)-stylopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
(S)-scoulerine + [reduced NADPH-hemoprotein reductase] + O2
Eschscholzia californica
-
(S)-nandinine + [oxidized NADPH-hemoprotein reductase] + H2O
enzyme does not catalyze the methylenedioxy bridge-formation in ring D of scoulerine but strictly recognizes ring A of the substrate
-
?
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2
Eschscholzia californica
-
(S)-tetrahydroberberine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
additional information
Eschscholzia californica
no substrates: columbamine, (R,S)-reticuline, (R,S)-norreticuline, (S)-N-methylcoclaurine, (S)-coclaurine, (R,S)-6-O-methylnorlaudanosoline, and magnoflorine
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Eschscholzia californica
-
root
30fold higher expression than in leaf
Eschscholzia californica
-
stem
-
Eschscholzia californica
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(R,S)-cheilanthifoline + [reduced NADPH-hemoprotein reductase] + O2
-
673579
Eschscholzia californica
(R,S)-stylopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(R,S)-cheilanthifoline + [reduced NADPH-hemoprotein reductase] + O2
-
673579
Eschscholzia californica
stylopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(S)-scoulerine + [reduced NADPH-hemoprotein reductase] + O2
-
673579
Eschscholzia californica
(S)-nandinine + [oxidized NADPH-hemoprotein reductase] + H2O
enzyme does not catalyze the methylenedioxy bridge-formation in ring D of scoulerine but strictly recognizes ring A of the substrate
-
-
?
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2
-
673579
Eschscholzia californica
(S)-tetrahydroberberine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
no substrates: columbamine, (R,S)-reticuline, (R,S)-norreticuline, (S)-N-methylcoclaurine, (S)-coclaurine, (R,S)-6-O-methylnorlaudanosoline, and magnoflorine
673579
Eschscholzia californica
?
-
-
-
-
Expression
Organism
Commentary
Expression
Eschscholzia californica
treatment with methyl jasmonate induces expression of both CYP719A2 and CYP719A3
up
Expression (protein specific)
Organism
Commentary
Expression
Eschscholzia californica
treatment with methyl jasmonate induces expression of both CYP719A2 and CYP719A3
up
Other publictions for EC 1.14.19.73
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
673579
Ikezawa
Molecular cloning and characte ...
Eschscholzia californica
FEBS J.
274
1019-1035
2007
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