BRENDA - Enzyme Database show
show all sequences of 1.14.19.74

Formation of two methylenedioxy bridges by a Sesamum CYP81Q protein yielding a furofuran lignan, (+)-sesamin

Ono, E.; Nakai, M.; Fukui, Y.; Tomimori, N.; Fukuchi-Mizutani, M.; Saito, M.; Satake, H.; Tanaka, T.; Katsuta, M.; Umezawa, T.; Tanaka, Y.; Proc. Natl. Acad. Sci. USA 103, 10116-10121 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed heterologously in yeast
Sesamum indicum
expressed heterologously in yeast
Sesamum radiatum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0102
-
(+)-pinoresinol
pH 7.4, 30°C
Sesamum indicum
0.0117
-
(+)-piperitol
pH 7.4, 30°C
Sesamum indicum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
cytoplasmic surface
Sesamum indicum
5783
-
endoplasmic reticulum
cytoplasmic surface
Sesamum radiatum
5783
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(+)-pinoresinol + [reduced NADPH-hemoprotein reductase]l + O2
Sesamum indicum
-
(+)-piperitol + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
?
(+)-pinoresinol + [reduced NADPH-hemoprotein reductase]l + O2
Sesamum radiatum
-
(+)-piperitol + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
?
(+)-piperitol + [reduced NADPH-hemoprotein reductase] + O2
Sesamum indicum
-
(+)-sesamin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
(+)-piperitol + [reduced NADPH-hemoprotein reductase] + O2
Sesamum radiatum
-
(+)-sesamin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sesamum indicum
Q33DY0
-
-
Sesamum radiatum
Q33DX9
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Sesamum indicum
-
additional information
the enzyme is not expressed in petals, or stems
Sesamum indicum
-
additional information
the enzyme is not expressed in leaves, stems, seed pods, or flowers
Sesamum radiatum
-
seed
expressed substantially in the later phases of seed development and then decreases before seed maturation is complete
Sesamum indicum
-
seed
-
Sesamum radiatum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-pinoresinol + [reduced NADPH-hemoprotein reductase] + O2
-
749158
Sesamum indicum
(+)-piperitol + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
(+)-pinoresinol + [reduced NADPH-hemoprotein reductase]l + O2
-
749158
Sesamum indicum
(+)-piperitol + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
(+)-pinoresinol + [reduced NADPH-hemoprotein reductase]l + O2
-
749158
Sesamum radiatum
(+)-piperitol + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
(+)-piperitol + [reduced NADPH-hemoprotein reductase] + O2
-
749158
Sesamum indicum
(+)-sesamin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
-
(+)-piperitol + [reduced NADPH-hemoprotein reductase] + O2
-
749158
Sesamum radiatum
(+)-sesamin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
-
additional information
neither (+)-sesamolinol nor (+)-phillygenin is used as substrate
749158
Sesamum radiatum
?
-
-
-
-
additional information
the enzyme does not catalyze (+)-sesamolin biosynthesis from (+)-sesamolinol
749158
Sesamum indicum
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P-450
a cytochrome P-450 (heme-thiolate) protein
Sesamum indicum
cytochrome P-450
a cytochrome P-450 (heme-thiolate) protein
Sesamum radiatum
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed heterologously in yeast
Sesamum indicum
expressed heterologously in yeast
Sesamum radiatum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P-450
a cytochrome P-450 (heme-thiolate) protein
Sesamum indicum
cytochrome P-450
a cytochrome P-450 (heme-thiolate) protein
Sesamum radiatum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0102
-
(+)-pinoresinol
pH 7.4, 30°C
Sesamum indicum
0.0117
-
(+)-piperitol
pH 7.4, 30°C
Sesamum indicum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
cytoplasmic surface
Sesamum indicum
5783
-
endoplasmic reticulum
cytoplasmic surface
Sesamum radiatum
5783
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(+)-pinoresinol + [reduced NADPH-hemoprotein reductase]l + O2
Sesamum indicum
-
(+)-piperitol + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
?
(+)-pinoresinol + [reduced NADPH-hemoprotein reductase]l + O2
Sesamum radiatum
-
(+)-piperitol + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
?
(+)-piperitol + [reduced NADPH-hemoprotein reductase] + O2
Sesamum indicum
-
(+)-sesamin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
(+)-piperitol + [reduced NADPH-hemoprotein reductase] + O2
Sesamum radiatum
-
(+)-sesamin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Sesamum indicum
-
additional information
the enzyme is not expressed in petals, or stems
Sesamum indicum
-
additional information
the enzyme is not expressed in leaves, stems, seed pods, or flowers
Sesamum radiatum
-
seed
expressed substantially in the later phases of seed development and then decreases before seed maturation is complete
Sesamum indicum
-
seed
-
Sesamum radiatum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-pinoresinol + [reduced NADPH-hemoprotein reductase] + O2
-
749158
Sesamum indicum
(+)-piperitol + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
(+)-pinoresinol + [reduced NADPH-hemoprotein reductase]l + O2
-
749158
Sesamum indicum
(+)-piperitol + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
(+)-pinoresinol + [reduced NADPH-hemoprotein reductase]l + O2
-
749158
Sesamum radiatum
(+)-piperitol + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
(+)-piperitol + [reduced NADPH-hemoprotein reductase] + O2
-
749158
Sesamum indicum
(+)-sesamin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
-
(+)-piperitol + [reduced NADPH-hemoprotein reductase] + O2
-
749158
Sesamum radiatum
(+)-sesamin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
-
additional information
neither (+)-sesamolinol nor (+)-phillygenin is used as substrate
749158
Sesamum radiatum
?
-
-
-
-
additional information
the enzyme does not catalyze (+)-sesamolin biosynthesis from (+)-sesamolinol
749158
Sesamum indicum
?
-
-
-
-
Expression
Organism
Commentary
Expression
Sesamum indicum
expressed substantially in the later phases of seed development and then decreases before seed maturation is complete
up
General Information
General Information
Commentary
Organism
metabolism
the enzyme is involved in furofuran lignan biosynthesis
Sesamum indicum
metabolism
the enzyme is involved in furofuran lignan biosynthesis
Sesamum radiatum
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is involved in furofuran lignan biosynthesis
Sesamum indicum
metabolism
the enzyme is involved in furofuran lignan biosynthesis
Sesamum radiatum
Expression (protein specific)
Organism
Commentary
Expression
Sesamum indicum
expressed substantially in the later phases of seed development and then decreases before seed maturation is complete
up
Other publictions for EC 1.14.19.74
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748932
Ono
Formation of a methylenedioxy ...
Sesamum indicum
Plant Cell Physiol.
59
2278-2287
2018
-
-
1
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
748900
Noguchi
-
Mode-of-action and evolution ...
Sesamum indicum
Plant Biotechnol.
31
493-503
2014
-
-
1
-
2
-
4
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
1
1
-
-
-
-
1
1
-
2
-
-
4
1
-
-
-
1
2
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
749158
Ono
Formation of two methylenedio ...
Sesamum indicum, Sesamum radiatum
Proc. Natl. Acad. Sci. USA
103
10116-10121
2006
-
-
2
-
-
-
-
2
2
-
-
4
-
8
-
-
-
-
-
5
-
-
7
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
2
-
-
-
-
-
-
2
2
-
-
4
-
-
-
-
-
5
-
-
7
-
-
-
-
-
-
-
-
-
1
2
2
1
-
-