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Literature summary for 1.14.19.B8 extracted from

  • Lin, S.; van Lanen, S.G., Shen, B.
    Regiospecific chlorination of (S)-beta-tyrosyl-S-carrier protein catalyzed by SgcC3 in the biosynthesis of the enediyne antitumor antibiotic C-1027 (2007), J. Am. Chem. Soc., 129, 12432-12438.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.19.B8

Cloned(Commentary)

Cloned (Comment) Organism
-
 Streptomyces globisporus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-(3S)-beta-tyrosyl-[peptidyl-carrier-protein SgcC2]-L-cysteine + 2 FADH2 + 2 H+ + Cl- + O2 Streptomyces globisporus the enzyme is involved in the biosynthesis of the (S)-3-chloro-5-hydroxy-beta-tyrosine moiety prior to incorporation into the chromoprotein antitumor antibiotic C-1027 S-(S3)-3-chloro-beta-tyrosyl-[peptidyl-carrier-protein SgcC2]-L-cysteine + FAD + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces globisporus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces globisporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme does not catalyze fluorination or iodination. The enzyme can not utilize 3-hydroxy-beta-tyrosyl-S-SgcC2 as a substrate Streptomyces globisporus ?
-
 ?
S-(3R)-beta-tyrosyl-[peptidyl-carrier-protein SgcC2]-L-cysteine + 2 FADH2 + 2 H+ + Cl- + O2
-
 Streptomyces globisporus S-(3R)-3-chloro-beta-tyrosyl-[peptidyl-carrier-protein SgcC2]-L-cysteine + FAD + 2 H2O
-
 ?
S-(3S)-beta-tyrosyl-[peptidyl-carrier-protein SgcC2]-L-cysteine + 2 FADH2 + 2 H+ + Br- + O2 the enzyme can also efficiently catalyzes bromination but not fluorination or iodination Streptomyces globisporus S-(3S)-3-bromo-beta-tyrosyl-[peptidyl-carrier-protein SgcC2]-L-cysteine + FAD + 2 H2O
-
 ?
S-(3S)-beta-tyrosyl-[peptidyl-carrier-protein SgcC2]-L-cysteine + 2 FADH2 + 2 H+ + Cl- + O2 the enzyme is involved in the biosynthesis of the (S)-3-chloro-5-hydroxy-beta-tyrosine moiety prior to incorporation into the chromoprotein antitumor antibiotic C-1027 Streptomyces globisporus S-(S3)-3-chloro-beta-tyrosyl-[peptidyl-carrier-protein SgcC2]-L-cysteine + FAD + 2 H2O
-
 ?
S-(3S)-beta-tyrosyl-[peptidyl-carrier-protein SgcC2]-L-cysteine + 2 FADH2 + 2 H+ + Cl- + O2
-
 Streptomyces globisporus S-(3S)-3-chloro-beta-tyrosyl-[peptidyl-carrier-protein SgcC2]-L-cysteine + FAD + 2 H2O
-
 ?

Synonyms

Synonyms Comment Organism
SgcC3
-
 Streptomyces globisporus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Streptomyces globisporus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Streptomyces globisporus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.5 pH 5.5: about 15% of maximal activity, pH 7.5: about 65% of maximal activity Streptomyces globisporus

General Information

General Information Comment Organism
physiological function the enzyme is involved in the biosynthesis of the (S)-3-chloro-5-hydroxy-beta-tyrosine moiety prior to incorporation into the chromoprotein antitumor antibiotic C-1027 Streptomyces globisporus