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Literature summary for 1.14.20.1 extracted from

  • Cortes, J.; Martin, J.F.; Castro, J.M.; Laiz, L.; Liras, P.
    Purification and characterization of a 2-oxoglutarate-linked ATP-independent deacetoxycephalosporin C synthase of Streptomyces lactamdurans (1987), J. Gen. Microbiol., 133, 3165-3174.
    View publication on PubMed
Search for more literature for 1.14.20.1

General Stability

General Stability Organism
increasing ionic strength to 100 decreases stability of the enzyme Amycolatopsis lactamdurans
the enzyme is very unstable and can be partially stabilized in 25 mM Tris-HCl, pH 9.0, in the presence of 0.1 mM DTT Amycolatopsis lactamdurans

Inhibitors

Inhibitors Comment Organism Structure
2-oxobutyrate 0.1 mM, complete inhibition Amycolatopsis lactamdurans
3-oxoadipate 0.1 mM, 56% inhibition Amycolatopsis lactamdurans
Co2+ 1 mM, 79% inhibition Amycolatopsis lactamdurans
Cu2+ 1 mM, complete inhibition Amycolatopsis lactamdurans
phosphate
-
 Amycolatopsis lactamdurans
Zn2+ 1 mM, 84% inhibition Amycolatopsis lactamdurans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 oxoglutarate
-
 Amycolatopsis lactamdurans
0.052
-
 Penicillin N
-
 Amycolatopsis lactamdurans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required Amycolatopsis lactamdurans
Fe2+ Km: 0.071 mM Amycolatopsis lactamdurans
Fe3+ can replace Fe2+ under reducing conditions Amycolatopsis lactamdurans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 gel filtration Amycolatopsis lactamdurans
28000
-
 1 * 28000, SDS-PAGE Amycolatopsis lactamdurans

Organism

Organism UniProt Comment Textmining
Amycolatopsis lactamdurans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Amycolatopsis lactamdurans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Amycolatopsis lactamdurans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with isopenicillin N or 6-aminopenicillanic acid Amycolatopsis lactamdurans ?
-
 ?
penicillin G + 2-oxoglutarate + O2 no activity Amycolatopsis lactamdurans phenylacetyl-7-aminodeacetoxy cephalosporanic acid + succinate + CO2 + H2O
-
 ?
penicillin N + 2-oxoglutarate + O2 oxoglutarate analogs are not used as substrate Amycolatopsis lactamdurans deacetoxycephalosporin C + succinate + CO2 + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 28000, SDS-PAGE Amycolatopsis lactamdurans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 30
-
 Amycolatopsis lactamdurans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 40 20°C: 15% of maximal activity, 35°C: 15% of maximal activity Amycolatopsis lactamdurans
20 35 20°C: 90% of maximal activity, 35°C: 90% of maximal activity Amycolatopsis lactamdurans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 60 min, stable Amycolatopsis lactamdurans
30
-
 60 min, 25% loss of activity Amycolatopsis lactamdurans
65
-
 60 min, complete inactivation Amycolatopsis lactamdurans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 11
-
 Amycolatopsis lactamdurans

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 4°C, 120 h, 80% loss of activity Amycolatopsis lactamdurans
8
-
 4°C, 120 h, 70% loss of activity Amycolatopsis lactamdurans
9
-
 4°C, 120 h, 35% loss of activity Amycolatopsis lactamdurans