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Literature summary for 1.14.20.1 extracted from

  • Sim, J.; Sim, T.S.
    Mutational evidence supporting the involvement of tripartite residues His183, Asp185, and His243 in Streptomyces clavuligerus deacetoxycephalosporin C synthase for catalysis (2000), Biosci. Biotechnol. Biochem., 64, 828-832.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes at high levels in Escherichia coli Streptomyces clavuligerus

Protein Variants

Protein Variants Comment Organism
D185L no detectable ring expansion activity Streptomyces clavuligerus
H183L no detectable ring expansion activity Streptomyces clavuligerus
H243L no detectable ring expansion activity Streptomyces clavuligerus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron non-heme iron-binding protein Streptomyces clavuligerus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
penicillin N + 2-oxoglutarate + O2 Streptomyces clavuligerus the enzyme is involved in catalyzing the biosynthesis of cephalosporins and cephamycin deacetoxycephalosporin C + succinate + CO2 + H2O
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Organism

Organism UniProt Comment Textmining
Streptomyces clavuligerus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
penicillin N + 2-oxoglutarate + O2
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Streptomyces clavuligerus deacetoxycephalosporin C + succinate + CO2 + H2O
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?
penicillin N + 2-oxoglutarate + O2 the enzyme is involved in catalyzing the biosynthesis of cephalosporins and cephamycin Streptomyces clavuligerus deacetoxycephalosporin C + succinate + CO2 + H2O
-
?