Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes at high levels in Escherichia coli | Streptomyces clavuligerus |
Protein Variants | Comment | Organism |
---|---|---|
D185L | no detectable ring expansion activity | Streptomyces clavuligerus |
H183L | no detectable ring expansion activity | Streptomyces clavuligerus |
H243L | no detectable ring expansion activity | Streptomyces clavuligerus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | non-heme iron-binding protein | Streptomyces clavuligerus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
penicillin N + 2-oxoglutarate + O2 | Streptomyces clavuligerus | the enzyme is involved in catalyzing the biosynthesis of cephalosporins and cephamycin | deacetoxycephalosporin C + succinate + CO2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces clavuligerus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
penicillin N + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | deacetoxycephalosporin C + succinate + CO2 + H2O | - |
? | |
penicillin N + 2-oxoglutarate + O2 | the enzyme is involved in catalyzing the biosynthesis of cephalosporins and cephamycin | Streptomyces clavuligerus | deacetoxycephalosporin C + succinate + CO2 + H2O | - |
? |