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Literature summary for 1.14.20.1 extracted from

  • Lin, B.; Fan, K.; Zhao, J.; Ji, J.; Wu, L.; Yang, K.; Tao, Y.
    Reconstitution of TCA cycle with DAOCS to engineer Escherichia coli into an efficient whole cell catalyst of penicillin G (2015), Proc. Natl. Acad. Sci. USA, 112, 9855-9859 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.20.1

Cloned(Commentary)

Cloned (Comment) Organism
gene efeE, cloning in Escherichia coli strain DH5alpha, recombinant expression in Escherichia coli strain K12 (BW25113) Streptomyces clavuligerus

Protein Variants

Protein Variants Comment Organism
additional information reconstitution of TCA cycle with enzyme DAOCS to engineer Escherichia coli into an efficient whole cell catalyst of penicillin G, overview. Cumulative effects of different combinations ofDELTAsucA,DELTAaceA,DELTApoxB::acs, and DELTAampC mutations on G- 7-aminodeacetoxycephalosporanic acid (G-7-ADCA) production. Additional engineering steps are taken to reduce the degradation of penicillin G and G-7-ADCA in the bioconversion process. Method optimization and evaluation, effects of deregulation of glyoxylate pathway and increased TCA flux on DAOCS catalyzed reaction, and effects of combining the beneficial mutations on G-7-ADCA production, overview Streptomyces clavuligerus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
penicillin N + 2-oxoglutarate + O2 Streptomyces clavuligerus
-
 deacetoxycephalosporin C + succinate + CO2 + H2O
-
 ?
penicillin N + 2-oxoglutarate + O2 Streptomyces clavuligerus ATCC 27064
-
 deacetoxycephalosporin C + succinate + CO2 + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces clavuligerus P18548
-
-
Streptomyces clavuligerus ATCC 27064 P18548
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
penicillin G + 2-oxoglutarate + O2
-
 Streptomyces clavuligerus 7-phenylacetylaminocephalosporanate + succinate + CO2 + H2O
-
 ?
penicillin G + 2-oxoglutarate + O2
-
 Streptomyces clavuligerus ATCC 27064 7-phenylacetylaminocephalosporanate + succinate + CO2 + H2O
-
 ?
penicillin N + 2-oxoglutarate + O2
-
 Streptomyces clavuligerus deacetoxycephalosporin C + succinate + CO2 + H2O
-
 ?
penicillin N + 2-oxoglutarate + O2
-
 Streptomyces clavuligerus ATCC 27064 deacetoxycephalosporin C + succinate + CO2 + H2O
-
 ?

Synonyms

Synonyms Comment Organism
DAOCS
-
 Streptomyces clavuligerus
deacetoxycephalosporin C synthase
-
 Streptomyces clavuligerus