Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | presence of reductant required | Hyoscyamus niger | |
dehydroascorbate | - |
Hyoscyamus niger | |
dithiothreitol | - |
Hyoscyamus niger | |
Fe2+ | - |
Hyoscyamus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4-dihydroxybenzoate | competitive inhibitor with respect to 2-oxoglutarate | Hyoscyamus niger | |
Cd2+ | 0.4 mM, 100% inhibition | Hyoscyamus niger | |
Co2+ | 0.4 mM, 100% inhibition | Hyoscyamus niger | |
Cu2+ | 0.4 mM, 100% inhibition | Hyoscyamus niger | |
EDTA | 0.1 mM, 67% inhibition | Hyoscyamus niger | |
Hg2+ | 0.4 mM, 96% inhibition | Hyoscyamus niger | |
Mn2+ | 0.4 mM, 95% inhibition | Hyoscyamus niger | |
Ni2+ | 0.4 mM, 96% inhibition | Hyoscyamus niger | |
nitroblue tetrazolium | - |
Hyoscyamus niger | |
Pyridine 2,4-dicarboxylate | competitive inhibitor with respect to 2-oxoglutarate | Hyoscyamus niger | |
Tiron | - |
Hyoscyamus niger | |
Zn2+ | 0.4 mM, 100% inhibition | Hyoscyamus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
(6S)-6beta-hydroxyhyoscyamine | pH 7.8, 30°C | Hyoscyamus niger | |
0.043 | - |
2-oxoglutarate | pH 7.8, 30°C | Hyoscyamus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
gel fitlration | Hyoscyamus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6,7-dehydrohyoscyamine + 2-oxoglutarate + O2 | Hyoscyamus niger | - |
scopolamine + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hyoscyamus niger | P24397 | - |
- |
Purification (Comment) | Organism |
---|---|
from root culture | Hyoscyamus niger |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | root culture | Hyoscyamus niger | - |
Storage Stability | Organism |
---|---|
-20°C, stable for at least 3 months | Hyoscyamus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(6S)-6beta-hydroxyhyoscyamine + 2-oxoglutarate + O2 | - |
Hyoscyamus niger | scopolamine + succinate + CO2 + H2O | - |
? | |
3-hydroxy-3-phenylpropionyltropine + 2-oxoglutarate + O2 | - |
Hyoscyamus niger | 3-hydroxy-3-phenylpropionyl-6-hydroxytropine + succinate + CO2 + H2O | - |
? | |
6,7-dehydrohyoscyamine + 2-oxoglutarate + O2 | - |
Hyoscyamus niger | scopolamine + succinate + CO2 | - |
? | |
apoatropine + 2-oxoglutarate + O2 | - |
Hyoscyamus niger | 6-hydroxyapoatropine + succinate + CO2 + H2O | - |
? | |
l-homatropine + 2-oxoglutarate + O2 | - |
Hyoscyamus niger | 6-hydroxyhomatropine + succinate + CO2 + H2O | - |
? | |
additional information | enzyme catalyzes both the hydroxylation of hyoscyamine, reaction of EC 1.14.11.11, and the epoxidation of 6beta-hydroxyhyoscyamine to generate scopolamine | Hyoscyamus niger | ? | - |
? | |
norhyoscyamine + 2-oxoglutarate + O2 | - |
Hyoscyamus niger | 6-hydroxynorhyoscyamine + succinate + CO2 + H2O | - |
? | |
phenylacetyltropine + 2-oxoglutarate + O2 | - |
Hyoscyamus niger | 6-hydroxyphenylacetyltropine + succinate + CO2 + H2O | - |
? | |
t-cinnamoyltropine + 2-oxoglutarate + O2 | - |
Hyoscyamus niger | t-cinnamoyl-6-hydroxytropine + succinate + CO2 + H2O | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Hyoscyamus niger |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
Pyridine 2,4-dicarboxylate | pH 7.8, 30°C | Hyoscyamus niger | |
0.09 | - |
3,4-dihydroxybenzoate | pH 7.8, 30°C | Hyoscyamus niger |