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Literature summary for 1.14.20.6 extracted from
- Cloning, characterization, and activity analysis of a flavonol synthase gene FtFLS1 and its association with flavonoid content in tartary buckwheat (2012), J. Agric. Food Chem., 60, 5161-5168.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene FtFLS1, DNA and amino acid sequence determination and analysis, phylogenetic tree, functional expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Fagopyrum tataricum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | required | Fagopyrum tataricum |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000, recombinant enzyme, SDS-PAGE | Fagopyrum tataricum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2R/3R)-dihydrokaempferol + 2-oxoglutarate + O2 | Fagopyrum tataricum | - |
kaempferol + succinate + CO2 + H2O | - |
? | |
| (2R/3R)-dihydroquercetin + 2-oxoglutarate + O2 | Fagopyrum tataricum | - |
quercetin + succinate + CO2 + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fagopyrum tataricum | F5BR59 | gene FtFLS1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Fagopyrum tataricum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | - |
Fagopyrum tataricum | - |
| flower bud | - |
Fagopyrum tataricum | - |
| leaf | highest enzyme expression, especially high at initial bloom | Fagopyrum tataricum | - |
| additional information | the overall expression levels are the highest in leaves and flowers, the enzyme shows a moderate level in stems, and lower expression in flower buds and immature seeds | Fagopyrum tataricum | - |
| seed | - |
Fagopyrum tataricum | - |
| stem | - |
Fagopyrum tataricum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0053 | - |
purified recombinant enzyme with substrate (2R/3R)-dihydrokaempferol, pH 5.0, 37°C | Fagopyrum tataricum |
| 0.019 | - |
purified recombinant enzyme with substrate (2R/3R)-dihydroquercetin, pH 5.0, 37°C | Fagopyrum tataricum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2R/3R)-dihydrokaempferol + 2-oxoglutarate + O2 | - |
Fagopyrum tataricum | kaempferol + succinate + CO2 + H2O | - |
? | |
| (2R/3R)-dihydroquercetin + 2-oxoglutarate + O2 | - |
Fagopyrum tataricum | quercetin + succinate + CO2 + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, recombinant enzyme, SDS-PAGE | Fagopyrum tataricum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FLS1 | - |
Fagopyrum tataricum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Fagopyrum tataricum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
assay at | Fagopyrum tataricum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the 2-oxoglutarate-dependent dioxygenase family | Fagopyrum tataricum |
| physiological function | the enzyme controls a key step in flavonol biosynthesis and might play a key role in rutin biosynthesis | Fagopyrum tataricum |
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