Literature summary for 1.14.20.7 extracted from

Copeland, R.A.; Davis, K.M.; Shoda, T.K.C.; Blaesi, E.J.; Boal, A.K.; Krebs, C.; Bollinger, J.M.
An iron(IV)-oxo intermediate initiating L-arginine oxidation but not ethylene production by the 2-oxoglutarate-dependent oxygenase, ethylene-forming enzyme (2021), J. Am. Chem. Soc., 143, 2293-2303 .

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Protein Variants

Protein Variants	Comment	Organism
D191E	the D191E variant degrades L-Arg and 2-oxoglutarateto pyrroline-5-carboxylate (again detected after reduction to proline and Fmoc derivatization) and succinate nearly stoichiometrically, with only about 5% of the cosubstrate being fragmented to ethylene	Pseudomonas savastanoi pv. phaseolicola

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	Fe2+-dependent enzyme	Pseudomonas savastanoi pv. phaseolicola

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-arginine + 2-oxoglutarate + O2	Pseudomonas savastanoi pv. phaseolicola	-	succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas savastanoi pv. phaseolicola	P32021	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-arginine + 2-oxoglutarate + O2	-	Pseudomonas savastanoi pv. phaseolicola	succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O	-	?
L-arginine + 2-oxoglutarate + O2	in the other reaction [EC 1.13.12.19, 2-oxoglutarate dioxygenase (ethene-forming)] the enzyme catalyses the dioxygenation of 2-oxoglutarate forming ethene and three molecules of carbon dioxide. An iron(IV)-oxo intermediate initiates L-arginine oxidation but not ethylene production by the 2-oxoglutarate-dependent oxygenase, ethylene-forming enzyme	Pseudomonas savastanoi pv. phaseolicola	succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O	-	?

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Release 2023.1 (January 2023)