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Literature summary for 1.14.99.26 extracted from

  • Sharma, M.L.; Kaul, S.M.; Shukla, O.P
    Metabolism of 2-hydroxypyridine by Bacillus brevis (INA) (1984), Biol. Mem., 9, 43-52.
No PubMed abstract available
Search for more literature for 1.14.99.26

General Stability

General Stability Organism
45 mM dithiothreitol affords partial protection against denaturation Brevibacillus brevis
50% glycerol affords partial protection against denaturation Brevibacillus brevis
dialysis of extracts in cold, 4°C, against phosphate buffer, pH 7.0, 0.05 M, for 4 h, 90% activity loss Brevibacillus brevis
enzyme is completely blocked by anaerobic conditions Brevibacillus brevis

Inhibitors

Inhibitors Comment Organism Structure
FMN 85% inhibition at 1.7 mM, 2 min preincubation Brevibacillus brevis
Na2As2O3 partial inhibition Brevibacillus brevis
NaF 53% inhibition at 1.7 mM, 5 min preincubation Brevibacillus brevis
NaN3 53% inhibition at 1.7 mM, 5 min preincubation Brevibacillus brevis
NiCl2 47% inhibition at 1.7 mM Brevibacillus brevis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Brevibacillus brevis 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-hydroxypyridine + NADH + O2 Brevibacillus brevis
-
 2,5-dihydroxypyridine + NAD+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Brevibacillus brevis
-
 isolated by enrichment on isonicotinic acid
-

Storage Stability

Storage Stability Organism
-10°C or 0°C, overnight, complete loss of activity Brevibacillus brevis
-20°C, Tris HCl buffer, pH 7.4, 0.05 M, 10% ethanol, 50% glycerol, 0.045 M dithiothreitol, 1 mM 2-mercaptoethanol, stable for 16 d Brevibacillus brevis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxypyridine + NADH + O2
-
 Brevibacillus brevis 2,5-dihydroxypyridine + NAD+ + H2O
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.2
-
 Brevibacillus brevis

pH Range

pH Minimum pH Maximum Comment Organism
6.3 8.5 half-maximal activity at pH 6.3 and pH 8.5 Brevibacillus brevis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Brevibacillus brevis
NADH
-
 Brevibacillus brevis
NADPH 2% activity of NADH Brevibacillus brevis