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Literature summary for 1.14.99.39 extracted from

  • Gilch, S.; Meyer, O.; Schmidt, I.
    A soluble form of ammonia monooxygenase in Nitrosomonas europaea (2009), Biol. Chem., 390, 863-873.
    View publication on PubMed
Search for more literature for 1.14.99.39

Inhibitors

Inhibitors Comment Organism Structure
Acetylene mechanism-based inhibitor, specifically interacts with catalytically active ammonia monooxygenase Nitrosomonas europaea

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm the enzyme resides in the cytoplasm of the bacteria in addition to its location in the membrane and is distributed approximately equally in both subcellular fractions Nitrosomonas europaea 5737
-
membrane the enzyme resides in the cytoplasm of the bacteria in addition to its location in the membrane and is distributed approximately equally in both subcellular fractions Nitrosomonas europaea 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ the enzyme contains Cu (9.4 mol per mol enzyme), Fe (3.9 mol per mol enzyme), and Zn (0.5 to 2.6 mol per mol enzyme) Nitrosomonas europaea
Iron the enzyme contains Cu (9.4 mol per mol enzyme), Fe (3.9 mol per mol enzyme), and Zn (0.5 to 2.6 mol per mol enzyme) Nitrosomonas europaea
Zinc the enzyme contains Cu (9.4 mol per mol enzyme), Fe (3.9 mol per mol enzyme), and Zn (0.5 to 2.6 mol per mol enzyme) Nitrosomonas europaea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 3 * 27000 (alpha-subunit, AmoA) + 3 * 42000 (beta-subunit, AmoB) + 3 * 24000 (gamma-subunit, cytochrome c1), soluble enzyme, SDS-PAGE Nitrosomonas europaea
27000
-
 3 * 27000 (alpha-subunit, AmoA) + 3 * 42000 (beta-subunit, AmoB) + 3 * 24000 (gamma-subunit, cytochrome c1), soluble enzyme, SDS-PAGE Nitrosomonas europaea
42000
-
 3 * 27000 (alpha-subunit, AmoA) + 3 * 42000 (beta-subunit, AmoB) + 3 * 24000 (gamma-subunit, cytochrome c1), soluble enzyme, SDS-PAGE Nitrosomonas europaea
283000
-
 non-denaturing gel electrophoresis Nitrosomonas europaea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ammonia + AH2 + O2 Nitrosomonas europaea
-
 NH2OH + A + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Nitrosomonas europaea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Nitrosomonas europaea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.089
-
 intact cells Nitrosomonas europaea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ammonia + AH2 + O2
-
 Nitrosomonas europaea NH2OH + A + H2O
-
 ?

Subunits

Subunits Comment Organism
More different from the beta-subunit of membrane-bound ammonia monooxygenase, the beta-subunit of soluble ammonia monooxygenase possesses an N-terminal signal sequence Nitrosomonas europaea
nonamer 3 * 27000 (alpha-subunit, AmoA) + 3 * 42000 (beta-subunit, AmoB) + 3 * 24000 (gamma-subunit, cytochrome c1), soluble enzyme, SDS-PAGE Nitrosomonas europaea

Cofactor

Cofactor Comment Organism Structure
cytochrome c1 the gamma-subunit of the alpha3beta3gamma3 enzyme is cytochrome c1 Nitrosomonas europaea