Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | the pyrimidine ring is cleaved directly by adding a pair of oxygen atoms and producing ureidoacrylate peracid. This odd metabolite, a strong and highly toxic oxidizing agent, is reduced spontaneously, albeit slowly, to ureidoacrylate | ? | - |
? | |
thymine + FMNH2 + O2 | Escherichia coli | - |
(Z)-2-methylureidoacrylate peracid + FMN | - |
? | |
uracil + FMNH2 + O2 | Escherichia coli | - |
(Z)-3-ureidoacrylate peracid + FMN | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P75898 | gene rutA, encoded in the rut gene cluster | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the pyrimidine ring is cleaved directly by adding a pair of oxygen atoms and producing ureidoacrylate peracid. This odd metabolite, a strong and highly toxic oxidizing agent, is reduced spontaneously, albeit slowly, to ureidoacrylate | Escherichia coli | ? | - |
? | |
thymine + FMNH2 + O2 | - |
Escherichia coli | (Z)-2-methylureidoacrylate peracid + FMN | - |
? | |
uracil + FMNH2 + O2 | - |
Escherichia coli | (Z)-3-ureidoacrylate peracid + FMN | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RutA | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
metabolism | RutA is the first enzyme of the pyrimidine catabolizing Rut pathway, the involved enzymes are encoded by the rut operon, including rutRABCDEFG and ydfG, overview. RutF functions as a flavin reductase that is required to regenerate the flavin mononucleotide, FMN, cofactor | Escherichia coli |
additional information | the Rut pathway degrades exogenous pyrimidines as the sole nitrogen source at room temperature but not at 37°C, a restriction that is apparently a consequence of an inadequate ability to remove toxic malonic semialdehyde at the higher temperature, RutE/YdfG function. The catabolic pathway is highly expressed even in the absence of exogenous substrate and at 37°C | Escherichia coli |