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Literature summary for 1.14.99.58 extracted from

  • Bhakta, M.N.; Wilks, A.
    The mechanism of heme transfer from the cytoplasmic heme binding protein PhuS to the delta-regioselective heme oxygenase of Pseudomonas aeruginosa (2006), Biochemistry, 45, 11642-11649.
    View publication on PubMedView publication on EuropePMC
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Protein Variants

Protein Variants Comment Organism
G125V single-phase kinetics of transfer of heme from heme-binding protein PhuS Pseudomonas aeruginosa
N19K/F117Y destabilization of heme within the protein, transfer of heme from heme binding protein PhuS is not affected and shows biphasic kinetics Pseudomonas aeruginosa
N19K/F117Y/K132A destabilization of heme within the protein, transfer of heme from heme binding protein PhuS is not affected and shows biphasic kinetics Pseudomonas aeruginosa
N19K/F117Y/K34N destabilization of heme within the protein, transfer of heme from heme binding protein PhuS is not affected and shows biphasic kinetics Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
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Posttranslational Modification

Posttranslational Modification Comment Organism
additional information heme transfer to the enzyme is independent of heme affinity and is mediated via a specific protein-protein interaction. A spin transition is involved during the transfer process with spin-state crossover occuring in the heme binding protein PhuS prior to the heme transfer step or alternatively occuring within the enzyme. Activation energies at pH 7.5 for the heme transfer from PhuS to enzyme are about 12-14 kcal per mol Pseudomonas aeruginosa