Application | Comment | Organism |
---|---|---|
synthesis | CotB3 can be employed to functionalize other diterpene olefins, such as (-)-casbene to yield sinularcasbane D | Streptomyces melanosporofaciens |
synthesis | efficient heterologous reconstitution of the cyclooctatin biosynthesis cluster by introduction of a novel, non-native Streptomyces-derived redox system (reductase/ferredoxin system from Streptomyces afghaniensis). The complete, heterologous biosynthesis cascade is established in Escherichia coli. The microbial production system allows for a 43fold increased cyclooctatin yield compared to the native producer Streptomyces melanosporofaciens | Streptomyces melanosporofaciens |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) | Streptomyces melanosporofaciens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclooctat-9-en-7-ol + reduced acceptor + O2 | Streptomyces melanosporofaciens | the enzyme is involved in the biosynthesis of cyclooctatin | cyclooctat-9-ene-5,7-diol + acceptor + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces melanosporofaciens | C9K1X6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclooctat-9-en-7-ol + reduced acceptor + O2 | the enzyme is involved in the biosynthesis of cyclooctatin | Streptomyces melanosporofaciens | cyclooctat-9-ene-5,7-diol + acceptor + H2O | - |
? | |
cyclooctat-9-en-7-ol + reduced acceptor + O2 | redox partner promiscuity of CotB3. The reductase/ferredoxin system from Streptomyces afghaniensis (AfR/Afx) is superior in activating the cylcooctatin-specific class I P450s CotB3/CotB4 compared to the conventional Pseudomonas putida reductase/ferredoxin system (PdR/Pdx) | Streptomyces melanosporofaciens | cyclooctat-9-ene-5,7-diol + acceptor + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CotB3 | - |
Streptomyces melanosporofaciens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Streptomyces melanosporofaciens |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the biosynthesis of cyclooctatin | Streptomyces melanosporofaciens |