Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glycerol | inhibits at 10%, activates at above 30% | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
LSD1 DNA and amino acid sequence determination and analysis, comparisons with other LSD isozymes of Arabidopsis thaliana and the human enzyme, recombinant His-tagged LSD1 expression in Escherichia coli | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glycerol | inhibits at 10%, activates at above 30% | Arabidopsis thaliana | |
KCl | 50 mM, 50% inhibition | Arabidopsis thaliana | |
MgCl2 | 75% inhibition at 50 mM | Arabidopsis thaliana | |
additional information | phosphorylation of the H3Ser10 residue totally abolishes the AtLSD1 demethylase activity toward the H3K4me1 peptide | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0089 | - |
[histone H3]-N6-methyl-L-lysine4 | pH 8.0, 22°C, recombinant LSD1 | Arabidopsis thaliana | |
0.0107 | - |
[histone H3]-N6,N6-dimethyl-L-lysine4 | pH 8.0, 22°C, recombinant LSD1 | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | nuclear localization signal at positions 517-534 of the LSD1 amino acid sequence | Arabidopsis thaliana | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[histone H3]-N6,N6-dimethyl-L-lysine4 + 2 2-oxoglutarate + 2 O2 | Arabidopsis thaliana | - |
[histone H3]-L-lysine4 + 2 succinate + 2 formaldehyde + 2 CO2 | - |
? | |
[histone H3]-N6-methyl-L-lysine4 + 2-oxoglutarate + O2 | Arabidopsis thaliana | - |
[histone H3]-L-lysine4 + succinate + formaldehyde + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q8VXV7 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
flavoprotein | - |
Arabidopsis thaliana |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged LSD1 from Escherichia coli by nickel and heparin affinity chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | high expression level | Arabidopsis thaliana | - |
leaf | rosette and cauline leaves | Arabidopsis thaliana | - |
additional information | semiquantitative RT-PCR tissue expression analysis | Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
silique | - |
Arabidopsis thaliana | - |
stem | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | LSD1 shows demethylase activity toward mono- and dimethylated Lys4 but not dimethylated Lys9 and Lys27 of histone 3, substrate and product identification by MALDI mass spectrometry. No LSD1 activity toward the H3K4me1-phosphoSer10 peptide | Arabidopsis thaliana | ? | - |
? | |
[histone H3]-N6,N6-dimethyl-L-lysine4 + 2 2-oxoglutarate + 2 O2 | - |
Arabidopsis thaliana | [histone H3]-L-lysine4 + 2 succinate + 2 formaldehyde + 2 CO2 | - |
? | |
[histone H3]-N6-methyl-L-lysine4 + 2-oxoglutarate + O2 | - |
Arabidopsis thaliana | [histone H3]-L-lysine4 + succinate + formaldehyde + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | LSD1 three-dimensional structure modelling on the basis of the human crystal structure as a template, overview | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
lysine-specific histone demethylase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
[histone H3]-N6-methyl-L-lysine4 | pH 8.0, 22°C, recombinant LSD1 | Arabidopsis thaliana | |
0.011 | - |
[histone H3]-N6,N6-dimethyl-L-lysine4 | pH 8.0, 22°C, recombinant LSD1 | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | - |
Arabidopsis thaliana |