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Literature summary for 1.14.99.66 extracted from

  • Ciccone, D.N.; Su, H.; Hevi, S.; Gay, F.; Lei, H.; Bajko, J.; Xu, G.; Li, E.; Chen, T.
    KDM1B is a histone H3K4 demethylase required to establish maternal genomic imprints (2009), Nature, 461, 415-418.
    View publication on PubMed
Search for more literature for 1.14.99.66

Cloned(Commentary)

Cloned (Comment) Organism
determination of expression of Aof1 wild-type and hetero- or homozygous mutants during development. Functional expression of Flag-tagged wild-type KDM1B and mutant KDM1B in nuclei of HEK 293T cells and NIH 3T3 cells Mus musculus

Protein Variants

Protein Variants Comment Organism
K667A point mutation in the amine oxidase domain, inactive mutant Mus musculus
additional information Aof1 hetero- or homozygous mutants, phenotypes, overview. Oocytes from KDM1B-deficient females show a substantial increase in H3K4 methylation and fail to set up the DNA methylation marks at four out of seven imprinted genes examined. KDM1B deficiency results in H3K4me2 elevation in oocytes and maternal-effect embryonic lethality Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Mus musculus 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus amine oxidase flavin-containing domain 1, AOF1, also called lysine demethylase 1B , KDM1B, is a protein related to the lysine demethylase KDM1or LSD1, it functions as a H3K4 demethylase and is required for de novo DNA methylation of some imprinted genes in oocytes ?
-
 ?
[histone H3]-N6,N6-dimethyl-L-lysine4 + 2 2-oxoglutarate + 2 O2 Mus musculus demethylation of H3K4 is critical for establishing the DNA methylation imprints during oogenesis [histone H3]-L-lysine4 + 2 succinate + 2 formaldehyde + 2 CO2
-
 ?
[histone H3]-N6,N6-methyl-L-lysine4 + 2-oxoglutarate + O2 Mus musculus demethylation of H3K4 is critical for establishing the DNA methylation imprints during oogenesis [histone H3]-L-lysine4 + succinate + formaldehyde + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
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-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Flag-tagged wild-type KDM1B and mutant KDM1B from HEK 293T cells to near homogeneity Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
 Mus musculus
-
germ cell male and female germ cells during gametogenesis Mus musculus
-
additional information Aof1 expression analysis during development, overview Mus musculus
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oocyte high expression level of lysine demethylase 1B, KDM1B Mus musculus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information amine oxidase flavin-containing domain 1, AOF1, also called lysine demethylase 1B , KDM1B, is a protein related to the lysine demethylase KDM1or LSD1, it functions as a H3K4 demethylase and is required for de novo DNA methylation of some imprinted genes in oocytes Mus musculus ?
-
 ?
additional information recombinant KDM1B shows no activity with H3K4me3, H3K9me2, H3K27me2 and H3K36me2 Mus musculus ?
-
 ?
[histone H3]-N6,N6-dimethyl-L-lysine4 + 2 2-oxoglutarate + 2 O2
-
 Mus musculus [histone H3]-L-lysine4 + 2 succinate + 2 formaldehyde + 2 CO2
-
 ?
[histone H3]-N6,N6-dimethyl-L-lysine4 + 2 2-oxoglutarate + 2 O2 demethylation of H3K4 is critical for establishing the DNA methylation imprints during oogenesis Mus musculus [histone H3]-L-lysine4 + 2 succinate + 2 formaldehyde + 2 CO2
-
 ?
[histone H3]-N6,N6-methyl-L-lysine4 + 2-oxoglutarate + O2
-
 Mus musculus [histone H3]-L-lysine4 + succinate + formaldehyde + CO2
-
 ?
[histone H3]-N6,N6-methyl-L-lysine4 + 2-oxoglutarate + O2 demethylation of H3K4 is critical for establishing the DNA methylation imprints during oogenesis Mus musculus [histone H3]-L-lysine4 + succinate + formaldehyde + CO2
-
 ?

Synonyms

Synonyms Comment Organism
amine oxidase flavin-containing domain 1
-
 Mus musculus
AOF1
-
 Mus musculus
histone H3 lysine 4 demethylase
-
 Mus musculus
histone H3K4 demethylase
-
 Mus musculus
KDM1B
-
 Mus musculus
lysine demethylase 1B
-
 Mus musculus

Cofactor

Cofactor Comment Organism Structure
FAD flavoenzyme Mus musculus

General Information

General Information Comment Organism
malfunction oocytes from KDM1B-deficient females show a substantial increase in H3K4 methylation and fail to set up the DNA methylation marks at four out of seven imprinted genes examined Mus musculus
physiological function amine oxidase flavin-containing domain 1, AOF1, is required for de novo DNA methylation of some imprinted genes in oocytes Mus musculus