Cloned (Comment) | Organism |
---|---|
coexpression of HA-tagged SALL4 and FLAG-tagged LSD1 in HEK-293 cells, coexpression of LSD1 and SALL4 proteins in mouse bone marrow hematopoietic stem and progenitor cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | hairpin RNA enzyme LSD1 knockdown, RNAi rescue experiments by expressing the human LSD1 cDNA | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
histone H3 N6,N6-dimethyl-L-lysine4 + 2-oxoglutarate + O2 | Mus musculus | - |
histone H3 N6-methyl-L-lysine4 + succinate + formaldehyde + CO2 | - |
? | |
histone H3 N6,N6-dimethyl-L-lysine4 + 2-oxoglutarate + O2 | Mus musculus C57/BL6J | - |
histone H3 N6-methyl-L-lysine4 + succinate + formaldehyde + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Mus musculus C57/BL6J | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bone marrow | - |
Mus musculus | - |
hematopoietic stem cell | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
histone H3 N6,N6-dimethyl-L-lysine4 + 2-oxoglutarate + O2 | - |
Mus musculus | histone H3 N6-methyl-L-lysine4 + succinate + formaldehyde + CO2 | - |
? | |
histone H3 N6,N6-dimethyl-L-lysine4 + 2-oxoglutarate + O2 | - |
Mus musculus C57/BL6J | histone H3 N6-methyl-L-lysine4 + succinate + formaldehyde + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
histone demethylase | - |
Mus musculus |
LSD1 | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | Cre-induced deletion of SALL4 in gene-targeted BM progenitors results in a remarkable decrease of SALL4-bound and LSD1-bound EBF1, accompanied by a 750fold increase of Lys4-dimethylated histon H3. Knockdown of LSD1 in bone marrow hematopoietic stem and progenitor cells leads to altered SALL4 downstream gene expression and increased cellular activity | Mus musculus |
physiological function | histone demethylase LSD1 is involved in SALL4 mediated transcriptional repression in hematopoietic stem cells, SALL4 and LSD1 co-occupy the same regions of GATA1, CEBPA, and TNF promoters, and SALL4 does dynamically recruit LSD1 to its target genes. LSD1 also appears to act as a central regulator for hematopoietic stem and progenitor cell proliferation and differentiation | Mus musculus |