Protein Variants | Comment | Organism |
---|---|---|
additional information | small interference RNA-mediated depletion of both LSD1 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tranylcypromine | an amino oxidase inhibitor, upregulates hTERT expression and telomerase activity concomitant with elevated H3K4me2 levels and H3 acetylation at the hTERT proximal promoter in cancer cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Homo sapiens | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[histone H3]-N6,N6-dimethyl-L-lysine 4 + 2-oxoglutarate + O2 | Homo sapiens | - |
[histone H3]-N6-methyl-L-lysine 4 + succinate + formaldehyde + CO2 | - |
? | |
[histone H3]-N6-methyl-L-lysine 4 + 2-oxoglutarate + O2 | Homo sapiens | - |
[histone H3]-L-lysine 4 + succinate + formaldehyde + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O60341 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
carcinoma cell | - |
Homo sapiens | - |
fibroblast | - |
Homo sapiens | - |
HL-60 cell | during the differentiation of leukemic HL60 cells, the decreased hTERT expression is accompanied by the LSD1 recruitment to the hTERT promoter | Homo sapiens | - |
leukemia cell | - |
Homo sapiens | - |
MRC-5 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the bifunctional enzyme catalyzes the demethylation of H3K9me2/me1 (EC 1.14.11.65) and H3K4me2/me1 | Homo sapiens | ? | - |
? | |
[histone H3]-N6,N6-dimethyl-L-lysine 4 + 2-oxoglutarate + O2 | - |
Homo sapiens | [histone H3]-N6-methyl-L-lysine 4 + succinate + formaldehyde + CO2 | - |
? | |
[histone H3]-N6-methyl-L-lysine 4 + 2-oxoglutarate + O2 | - |
Homo sapiens | [histone H3]-L-lysine 4 + succinate + formaldehyde + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KDM1A | - |
Homo sapiens |
LSD1 | - |
Homo sapiens |
lysine-specific demethylase 1 | - |
Homo sapiens |
More | see also EC 1.14.11.65 | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | in normal human fibroblasts with a tight hTERT repression, a pharmacological inhibition of LSD1 leads to a weak hTERT expression, and a robust induction of hTERT mRNA occurs when LSD1 and histone deacetylases are both inhibited. Small interference RNA-mediated depletion of both LSD1 and CoREST, a co-repressor in HDAC-containing complexes, synergistically activate hTERT transcription. In cancer cells, inhibition of LSD1 activity or knocking-down of its expression lead to significant increases in levels of hTERT mRNA and telomerase activity, phenotype, overview. LSD1 occupies the hTERT proximal promoter, and its depletion results in elevated dimethylation of histone H3-K4 accompanied by increased H3 acetylation locally in cancer cells | Homo sapiens |
physiological function | LSD1 catalyzes the demethylation of mono- and dimethylated histone H3-K4 and also H3-K9, it exhibits diverse transcriptional activities by mediating chromatin reconfiguration. LSD1 represses hTERT transcription via demethylating H3-K4 in normal and cancerous cells, and together with HDACs, participates in the establishment of a stable repression state of the hTERT gene in normal or differentiated malignant cells. Role for LSD1 in controlling hTERT transcription | Homo sapiens |