Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.99.68 extracted from

  • Zocher, G.; Winkler, R.; Hertweck, C.; Schulz, G.
    Structure and action of the N-oxygenase AurF from Streptomyces thioluteus (2007), J. Mol. Biol., 373, 65-74 .
    View publication on PubMed
Search for more literature for 1.14.99.68

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as a fusion with an N-terminal maltose-binding protein Streptomyces thioluteus

Crystallization (Commentary)

Crystallization (Comment) Organism
structure at 2.1 A resolution shows a homodimer with a binuclear manganese cluster and an iron content of about 15%. An additional histidine residue in the coordination sphere causes the preference for manganese over iron. There is no oxo-bridge Streptomyces thioluteus

Protein Variants

Protein Variants Comment Organism
F264A 105% of wild-type activity Streptomyces thioluteus
L202F 357% of wild-type activity Streptomyces thioluteus
L300W 80% of wild-type activity Streptomyces thioluteus
R96A complete loss of activity Streptomyces thioluteus
T100A 314% of wild-type activity Streptomyces thioluteus
T100L 14% of wild-type activity Streptomyces thioluteus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron iron content is about 15% Streptomyces thioluteus
Manganese crystal structure shows a binuclear manganese cluster Streptomyces thioluteus

Organism

Organism UniProt Comment Textmining
Streptomyces thioluteus Q70KH9
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0032
-
 23°C, pH 7.5 Streptomyces thioluteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-aminobenzoate + reduced acceptor + 2 O2
-
 Streptomyces thioluteus 4-nitrobenzoate + acceptor + 2 H2O
-
 ?

Subunits

Subunits Comment Organism
dimer crystal structure shows a homodimer Streptomyces thioluteus