Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | the in vitro enzymatic activity of AurF is best reconstituted in the presence of phenazine methosulfate and ascorbate | Streptomyces thioluteus | |
phenazine methosulfate | the in vitro enzymatic activity of AurF is best reconstituted in the presence of phenazine methosulfate and ascorbate | Streptomyces thioluteus |
Crystallization (Comment) | Organism |
---|---|
structure of AurF in the oxidized state, and the cocrystal structure of AurF with its product 4-nitrobenzoate | Streptomyces thioluteus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0052 | - |
4-Aminobenzoate | pH 7.4, 20°C | Streptomyces thioluteus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | the iron-to-enzyme ratio is 2.2, protein shows a high selectivity for iron | Streptomyces thioluteus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces thioluteus | Q70KH9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminobenzoate + reduced acceptor + 2 O2 | - |
Streptomyces thioluteus | 4-nitrobenzoate + acceptor + 2 H2O | - |
? | |
additional information | the yield of the final product 4-nitrobenzoate is 37.2% when equimoles of reductants phenazine methosulfate and 4-aminobenzoic acid and 4 equimoles of ascorbate are used | Streptomyces thioluteus | ? | - |
- |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.104 | - |
4-Aminobenzoate | pH 7.4, 20°C | Streptomyces thioluteus |
General Information | Comment | Organism |
---|---|---|
physiological function | AurF is a non-heme di-iron monooxygenase that catalyzes sequential oxidation of aminoarenes to nitroarenes via hydroxylamine and nitroso intermediates | Streptomyces thioluteus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
4-Aminobenzoate | pH 7.4, 20°C | Streptomyces thioluteus |