Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain JM109(DE3) | Saccharolobus solfataricus |
Crystallization (Comment) | Organism |
---|---|
purified mutant enzyme Y41F, hanging drop vapor diffusion method, 21°C, 1:1 mix of the reservoir solution containing 8% PEG 8000, 0.1 M Tris-HCl, pH 8.5, and the protein solution containing 1.45 mg/mL Y41F, 20 mM Tris-HCl, pH 7.8, and 1% glycerol, X-ray diffraction structure determination and analysis | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
H155Q | site-directed mutagenesis, the mutant shows a a slightly lower iron content, reduced heat stability, and a 2fold reduced activity compared to the wild-type enzyme | Saccharolobus solfataricus |
Y41F | site-directed mutagenesis, the mutant shows a a slightly lower iron content and a 17fold reduced activity compared to the wild-type enzyme, the mutant shows an uninterrupted hydrogen bond network | Saccharolobus solfataricus |
General Stability | Organism |
---|---|
SDS decreases the stability of SOD and accelerates enzyme inactivation | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaN3 | inhibits the enzyme mutants Y41F and H155Q, but not the wild-type enzyme | Saccharolobus solfataricus | |
PMSF | irreversible inhibition by binding to active site Tyr41 | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | Fe-type SOD | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
4 * 24000, wild-type and mutant enzymes, SDS-PAGE | Saccharolobus solfataricus |
87900 | - |
wild-type and mutant enzymes, gel filtration | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
387 | - |
purified mutant Y41F | Saccharolobus solfataricus |
3980 | - |
purified mutant H155Q | Saccharolobus solfataricus |
6720 | - |
purified wild-type enzyme | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | covalent modification of the conserved Tyr41 in the active site, Tyr41 and His155 are involved in catalysis, hydrogen bond network including three solvent molecules connecting the iron-ligating hydroxide ion via H155 with F41 and H37, Y41 and H155 are important for the structural and functional properties of SOD, overview | Saccharolobus solfataricus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 24000, wild-type and mutant enzymes, SDS-PAGE | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
SOD | - |
Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.4 | assay at | Saccharolobus solfataricus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharolobus solfataricus | isoelectric focusing | - |
8.4 |