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Literature summary for 1.15.1.1 extracted from

  • Shijin, E.; Guo, F.; Liu, S.; Chen, J.; Wang, Y.; Li, D.
    Purification, characterization, and molecular cloning of a thermostable superoxide dismutase from Thermoascus aurantiacus (2007), Biosci. Biotechnol. Biochem., 71, 1090-1093.
    View publication on PubMed
Search for more literature for 1.15.1.1

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparison Thermoascus aurantiacus

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ a Cu,Zn-SOD Thermoascus aurantiacus
Zn2+ a Cu,Zn-SOD Thermoascus aurantiacus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16800
-
 2 * 16800, SDS-PAGE Thermoascus aurantiacus
33200
-
 gel filtration Thermoascus aurantiacus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
O2.- + H+ Thermoascus aurantiacus
-
 O2 + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Thermoascus aurantiacus Q1HDV5 var. levisporus
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme to homogeneity by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography Thermoascus aurantiacus

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
150
-
 purified native enzyme Thermoascus aurantiacus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O2.- + H+
-
 Thermoascus aurantiacus O2 + H2O2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 16800, SDS-PAGE Thermoascus aurantiacus

Synonyms

Synonyms Comment Organism
Cu,Zn-SOD
-
 Thermoascus aurantiacus
SOD
-
 Thermoascus aurantiacus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 purified native enzyme, 60 min, completely stable Thermoascus aurantiacus
60
-
 purified native enzyme, 95% remaining activity after 60 min Thermoascus aurantiacus
70
-
 purified native enzyme, 55% remaining activity after 60 min Thermoascus aurantiacus
80
-
 purified native enzyme, inactivation after 60 min Thermoascus aurantiacus
90
-
 purified native enzyme, inactivation after 50 min Thermoascus aurantiacus