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Literature summary for 1.15.1.1 extracted from

  • Wang, X.; Yang, H.; Ruan, L.; Liu, X.; Li, F.; Xu, X.
    Cloning and characterization of a thermostable superoxide dismutase from the thermophilic bacterium Rhodothermus sp. XMH10 (2008), J. Ind. Microbiol. Biotechnol., 35, 133-139.
    View publication on PubMed
Search for more literature for 1.15.1.1

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determnination and analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) Rhodothermus sp.

Inhibitors

Inhibitors Comment Organism Structure
H2O2 inhibition at 10 mM, the Fe-SOD is less sensitive Rhodothermus sp.
KCN inhibition at 10 mM, the Fe-SOD is less sensitive Rhodothermus sp.
NaN3 inhibition at 5 mM, the Fe-SOD is less sensitive Rhodothermus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ a Fe-SOD Rhodothermus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23600
-
 2 * 23600, about, recombinant enzyme, sequence calcualtion, 2 * 25000, recombinant enzyme, SDS-PAGE Rhodothermus sp.
25000
-
 2 * 23600, about, recombinant enzyme, sequence calcualtion, 2 * 25000, recombinant enzyme, SDS-PAGE Rhodothermus sp.
52000
-
 recombinant enzyme, gel filtration Rhodothermus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
O2.- + H+ Rhodothermus sp.
-
 O2 + H2O2
-
 ?
O2.- + H+ Rhodothermus sp. XMH10
-
 O2 + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodothermus sp.
-
-
-
Rhodothermus sp. XMH10
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Rhodothermus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O2.- + H+
-
 Rhodothermus sp. O2 + H2O2
-
 ?
O2.- + H+
-
 Rhodothermus sp. XMH10 O2 + H2O2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 23600, about, recombinant enzyme, sequence calcualtion, 2 * 25000, recombinant enzyme, SDS-PAGE Rhodothermus sp.

Synonyms

Synonyms Comment Organism
SOD
-
 Rhodothermus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 70 recombinant enzyme Rhodothermus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 12 h, purified recombinant enzyme, 90% remaining activity Rhodothermus sp.
80
-
 purified recombinant enzyme, 40% remaining activity after 30 min, complete inactivation after 4 h Rhodothermus sp.
90
-
 purified recombinant enzyme, 10% remaining activity after 30 min Rhodothermus sp.
100
-
 purified recombinant enzyme, complete inactivation after 30 min Rhodothermus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 recombinant enzyme Rhodothermus sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 12 inactivation at pH 2.0, shrap drop of activity below pH 4.0, high activity at pH 4.0-pH 5.0, about 70% of maximal activity at pH 6.0-12.0 Rhodothermus sp.

pI Value

Organism Comment pI Value Maximum pI Value
Rhodothermus sp. sequence calcualtion
-
 5.54