Cloned (Comment) | Organism |
---|---|
gene sodA, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strains DH5alpha and BW19851, complementation of an enzyme-deficient mutant strain | Providencia alcalifaciens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of an enzyme-deletion mutant, the mutant strain is less virulent in mice than the wild-type strain or the complemented strain, overview | Providencia alcalifaciens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | a Mn-SOD | Providencia alcalifaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
x * 25000, recombinant enzyme, SDS-PAGE | Providencia alcalifaciens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O2.- + H+ | Providencia alcalifaciens | the enzyme is important in defense of cells against oxidative stress | O2 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Providencia alcalifaciens | - |
gene sodA | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography, to homogeneity | Providencia alcalifaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O2.- + H+ | - |
Providencia alcalifaciens | O2 + H2O2 | - |
? | |
O2.- + H+ | the enzyme is important in defense of cells against oxidative stress | Providencia alcalifaciens | O2 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 25000, recombinant enzyme, SDS-PAGE | Providencia alcalifaciens |
Synonyms | Comment | Organism |
---|---|---|
manganese superoxide dismutase | - |
Providencia alcalifaciens |
Mn-SOD | - |
Providencia alcalifaciens |