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Literature summary for 1.15.1.1 extracted from

  • Chen, X.; Kodama, T.; Iida, T.; Honda, T.
    Demonstration and characterization of manganese superoxide dismutase of Providencia alcalifaciens (2007), Microbiol. Immunol., 51, 951-961.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene sodA, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strains DH5alpha and BW19851, complementation of an enzyme-deficient mutant strain Providencia alcalifaciens

Protein Variants

Protein Variants Comment Organism
additional information construction of an enzyme-deletion mutant, the mutant strain is less virulent in mice than the wild-type strain or the complemented strain, overview Providencia alcalifaciens

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ a Mn-SOD Providencia alcalifaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
x * 25000, recombinant enzyme, SDS-PAGE Providencia alcalifaciens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
O2.- + H+ Providencia alcalifaciens the enzyme is important in defense of cells against oxidative stress O2 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Providencia alcalifaciens
-
gene sodA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography, to homogeneity Providencia alcalifaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O2.- + H+
-
Providencia alcalifaciens O2 + H2O2
-
?
O2.- + H+ the enzyme is important in defense of cells against oxidative stress Providencia alcalifaciens O2 + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 25000, recombinant enzyme, SDS-PAGE Providencia alcalifaciens

Synonyms

Synonyms Comment Organism
manganese superoxide dismutase
-
Providencia alcalifaciens
Mn-SOD
-
Providencia alcalifaciens