Literature summary for 1.15.1.1 extracted from
Xie, X.; Ying, S.; Feng, M.
Characterization of a new Cu/Zn-superoxide dismutase from Beauveria bassiana and two site-directed mutations crucial to its antioxidation activity without chaperon (2010), Enzyme Microb. Technol., 46, 217-222.
No PubMed abstract available
Cloned(Commentary)
Cloned (Comment) |
Organism |
gene BbSod1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, subcloning in Escherichia coli strain DH5alpha, expression as His-tagged wild-type and mutant enzymes and as His-tagged Saccharomyces cerevisiae superoxide dismutase 1 copper chaperone-fusion enzyme, i.e. SOD1-Lys7, in Escherichia coli BL21(DE3) |
Beauveria bassiana |
Protein Variants
Protein Variants |
Comment |
Organism |
additional information |
construction of a chimeric SOD1 mutant fused to Saccharomyces cerevisiae superoxide dismutase 1 copper chaperone-fusion enzyme, Lys7, the chimeric mutant SOD1-Lys7 shows increased activirty compared to the wild-type SOD1 and SOD1 mutant P143S/P145L |
Beauveria bassiana |
P143S/P145L |
site-directed mutagenesis, gain-of-function, the mutant shows increased activirty compared to the wild-type SOD1 |
Beauveria bassiana |
Metals/Ions
Metals/Ions |
Comment |
Organism |
Structure |
Cu2+ |
a CuZn-superoxide dismutase |
Beauveria bassiana |
|
Zn2+ |
a CuZn-superoxide dismutase |
Beauveria bassiana |
|
Molecular Weight [Da]
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
45000 |
- |
x *17000, recombinant SOD1, SDS-PAGE, x * 45000, recombinant SOD1-Lys7, SDS-PAGE |
Beauveria bassiana |
Organism
Organism |
UniProt |
Comment |
Textmining |
Beauveria bassiana |
C7EXL1 |
gene BbSod1 |
- |
Beauveria bassiana 2860 |
C7EXL1 |
gene BbSod1 |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
recombinant His-tagged wild-type and mutant enzymes and His-tagged SOD1-Lys7 from Escherichia coli BL21(DE3) by nickel affinity chromatography |
Beauveria bassiana |
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
178.7 |
- |
recombinant wild-type SOD1, pH not specified in the publication, temperature not specified in the publication |
Beauveria bassiana |
1331 |
- |
recombinant SOD1-Lys7 chimera, pH not specified in the publication, temperature not specified in the publication |
Beauveria bassiana |
3788 |
- |
recombinant SOD1 mutant P143S/P145L, pH not specified in the publication, temperature not specified in the publication |
Beauveria bassiana |
Subunits
Subunits |
Comment |
Organism |
? |
x *17000, recombinant SOD1, SDS-PAGE, x * 45000, recombinant SOD1-Lys7, SDS-PAGE |
Beauveria bassiana |
Synonyms
Synonyms |
Comment |
Organism |
Cu/Zn-superoxide dismutase |
- |
Beauveria bassiana |
SOD1 |
- |
Beauveria bassiana |