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Literature summary for 1.15.1.1 extracted from
- Purification and characterization of a hyperthermostable Mn-superoxide dismutase from Thermus thermophilus HB27 (2011), Extremophiles, 15, 221-226.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli strain BL21 (DE3) | Thermus thermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | inhibits by 25% at 10 mM | Thermus thermophilus |  |
| Fe2+ | inhibits by 44% at 10 mM | Thermus thermophilus | |
| Hg2+ | inhibits by 35% at 10 mM | Thermus thermophilus | |
| K+ | inhibits by 14% at 10 mM | Thermus thermophilus | |
| Mg2+ | inhibits by 64% at 10 mM | Thermus thermophilus | |
| Ni2+ | inhibits by 57% at 10 mM | Thermus thermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | activates by 10% at 10 mM | Thermus thermophilus | |
| Cu2+ | activates by 16% at 10 mM | Thermus thermophilus | |
| Mn2+ | activates by 12% at 10 mM, Mn-containing superoxide dismutase | Thermus thermophilus | |
| Zn2+ | activates by 48% at 10 mM | Thermus thermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
4 * 24000, SDS-PAGE and gel filtration | Thermus thermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 O2.- + 2 H+ + | Thermus thermophilus | - |
O2 + H2O2 | - |
? | |
| 2 O2.- + 2 H+ + | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | - |
O2 + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | - |
- |
- |
| Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme 3.4fold from Escherichia coli strain BL21 (DE3) by two-stage ultrafiltration to 92.6% purity | Thermus thermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1418 | - |
purified recombinant enzyme, one unit of SOD activity is defined as the amount of enzyme required to inhibit the autooxidation of pyrogallol to 50%. pH 8.2, 37°C | Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 O2.- + 2 H+ + | - |
Thermus thermophilus | O2 + H2O2 | - |
? | |
| 2 O2.- + 2 H+ + | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | O2 + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 24000, SDS-PAGE and gel filtration | Thermus thermophilus |
| More | secondary structure analysis by CD spectroscopy, the enzyme has a high alpha-helical content of 70%, overview | Thermus thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Mn-containing superoxide dismutase | - |
Thermus thermophilus |
| Mn-SOD | - |
Thermus thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Thermus thermophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 115 | activity range, profile, overview | Thermus thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | 90 | purified recombinant enzyme, 60 min, stable | Thermus thermophilus |
| 100 | - |
purified recombinant enzyme, 57% activity remaining activity after 60 min, half-life is about 70 min | Thermus thermophilus |
| 110 | - |
purified recombinant enzyme, 20% activity remaining activity after 30 min | Thermus thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Thermus thermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 11 | pH-profile, overview. Outside the range of 5.0-7.0, activity is rapidly lost | Thermus thermophilus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Thermus thermophilus | recombinant enzyme, isoelectric focusing | - |
6.2 |
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