Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.15.1.1 extracted from

  • Isarankura-Na-Ayudhya, C.; Yainoy, S.; Tantimongcolwat, T.; Buelow, L.; Prachayasittikul, V.
    Engineering of a novel chimera of superoxide dismutase and Vitreoscilla hemoglobin for rapid detoxification of reactive oxygen species (2010), J. Biosci. Bioeng., 110, 633-637.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of recombinant chimera MnSOD-VHb in Escherichia coli strain BL21(DE3) Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information engineering of a novel chimera of human Mn-SOD and Vitreoscilla hemoglobin, i.e. MnSOD-VHb, for rapid detoxification of reactive oxygen species, the recombinant bifunctional enzyme possesses MnSOD and peroxidase-like activities. The greater antioxidant capability is possibly due to the close proximity between the active site of MnSOD and the heme moiety of VHb, synergistic functions of SOD and peroxidase, overview Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetics with artificial substrates, overview Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mn a Mn-SOD Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
4 * 22000, Mn-SOD, SDS-PAGE Homo sapiens
38000
-
2 * 38000, recombinant chimera MnSOD-VHb, SDS-PAGE Homo sapiens
76000
-
recombinant chimera MnSOD-VHb, gel filtration Homo sapiens
88000
-
Mn-SOD, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 O2.- + 2 H+ + Homo sapiens
-
O2 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant chimera MnSOD-VHb from Escherichia coli strain BL21(DE3) Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 O2.- + 2 H+ +
-
Homo sapiens O2 + H2O2
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 38000, recombinant chimera MnSOD-VHb, SDS-PAGE Homo sapiens
homotetramer 4 * 22000, Mn-SOD, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
manganese superoxide dismutase
-
Homo sapiens
MnSOD
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Homo sapiens

General Information

General Information Comment Organism
additional information the chimeric protein MnSOD-VHb is a dimer, in which the two subunits are associated via interaction between the SOD portions, since MnSOD monomers show a 40fold reduction in activity, but our chimeric MnSOD-VHb retained 84% activity compared to native MnSOD Homo sapiens