Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)pLys | Withania somnifera |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the purified recombinant enzyme shows a high degree of resistance to detergent, ethanol and protease digestion | Withania somnifera |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Withania somnifera | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | a Cu/Zn SOD, 0.83 ng atom Cu per mg protein | Withania somnifera | |
additional information | iron, manganese, and nickel contents are below the detection level | Withania somnifera | |
Zn2+ | a Cu/Zn SOD, 0.41 ng atom Zn per mg protein | Withania somnifera |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15960 | - |
2 * 15960, sequence calculation, 2 * 17000, recombinant enzyme, SDS-PAGE | Withania somnifera |
17000 | - |
2 * 15960, sequence calculation, 2 * 17000, recombinant enzyme, SDS-PAGE | Withania somnifera |
34000 | - |
recombinant enzyme, native PAGE and gel filtration | Withania somnifera |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Withania somnifera | G0WP59 | RSS-30 chemotype | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | the enzyme sequence shows the presence of putative sites of disulphide bonds | Withania somnifera |
no glycoprotein | the enzyme sequence lacks any putative glycosylation sites | Withania somnifera |
phosphoprotein | the enzyme sequence shows the presence of putative sites of phosphorylation | Withania somnifera |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3)pLys to near homogeneity | Withania somnifera |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4200 | - |
purified recombinant enzyme, pH and temperature not specified in the publication | Withania somnifera |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 15960, sequence calculation, 2 * 17000, recombinant enzyme, SDS-PAGE | Withania somnifera |
Synonyms | Comment | Organism |
---|---|---|
Cu/Zn SOD | - |
Withania somnifera |
Cu/Zn superoxide dismutase | - |
Withania somnifera |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
purified recombinant enzyme, 1 h, 90% activity remaining | Withania somnifera |
90 | - |
purified recombinant enzyme, 1 h, 70% activity remaining | Withania somnifera |
95 | - |
purified recombinant enzyme, 4.68 h, 50% activity remaining | Withania somnifera |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.5 | 11 | purified recombinnat enzyme, stable in a broad range | Withania somnifera |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Withania somnifera | sequence calculation | - |
5.33 |