Cloned (Comment) | Organism |
---|---|
isozyme SODI, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis | Aspergillus glaucus |
isozyme SODII, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis | Aspergillus glaucus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | a Cu/ZnSOD | Aspergillus glaucus | |
additional information | presence of Cu and Zn is confirmed by inductively coupled plasma mass spectrometry, metal content analysis reveals a 1/1 molar ratio (metal/protein) for Cu and Zn, without significant amounts of Fe, Mn or Ni | Aspergillus glaucus | |
additional information | presence of Cu and Zn is confirmed by inductively coupled plasma mass spectrometry, metal content analysis reveals for SODI molar ratios (metal/protein) for Cu = 0.7 and Zn = 0.4, and for Mn = 0.16 | Aspergillus glaucus | |
Zn2+ | a Cu/ZnSOD | Aspergillus glaucus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 superoxide + 2 H+ | Aspergillus glaucus | - |
O2 + H2O2 | - |
? | |
2 superoxide + 2 H+ | Aspergillus glaucus 363/NBIMCC 8861 | - |
O2 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus glaucus | - |
- |
- |
Aspergillus glaucus | A0A1L9VGR0 | - |
- |
Aspergillus glaucus 363/NBIMCC 8861 | - |
- |
- |
Aspergillus glaucus 363/NBIMCC 8861 | A0A1L9VGR0 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | glycosylation analysis, overview | Aspergillus glaucus |
no glycoprotein | glycosylation analysis, overview | Aspergillus glaucus |
Purification (Comment) | Organism |
---|---|
native isozyme SODI by 23fold gel filtration, hydrophobic interaction chromatography, and anion-exchange chromatography | Aspergillus glaucus |
native isozyme SODII by 153fold gel filtration, hydrophobic interaction chromatography, and anion-exchange chromatography to homogeneity | Aspergillus glaucus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | submerged cultivation | Aspergillus glaucus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
623 | - |
purified native isozyme SODI, pH and temperature not specified in the publication | Aspergillus glaucus |
4000 | - |
purified native isozyme SODII, pH and temperature not specified in the publication | Aspergillus glaucus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 superoxide + 2 H+ | - |
Aspergillus glaucus | O2 + H2O2 | - |
? | |
2 superoxide + 2 H+ | - |
Aspergillus glaucus 363/NBIMCC 8861 | O2 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 15835, mass spectrometry and dynamic light scattering | Aspergillus glaucus |
? | x * 38159, mass spectrometry and dynamic light scattering | Aspergillus glaucus |
Synonyms | Comment | Organism |
---|---|---|
cold-active superoxide dismutase | - |
Aspergillus glaucus |
Cu/Zn superoxide dismutase | - |
Aspergillus glaucus |
Cu/Zn-SODI | - |
Aspergillus glaucus |
Cu/Zn-SODII | - |
Aspergillus glaucus |
SOD | - |
Aspergillus glaucus |
SODI | - |
Aspergillus glaucus |
SODII | - |
Aspergillus glaucus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
SODI displays a Tm value of 54°C and therefore its structure is rather labile to temperature | Aspergillus glaucus |
additional information | - |
SODII displays a Tm value of 80°C and therefore its structure is rather stable to temperature | Aspergillus glaucus |
General Information | Comment | Organism |
---|---|---|
evolution | the N-terminal amino acid sequence of Cu/Zn-SODII reveals a high degree of structural homology with Cu/Zn-SOD from other fungi, including Aspergillus species | Aspergillus glaucus |
physiological function | the major isoenzyme is Cu/Zn-SODII, the minor Cu/Zn-SODI | Aspergillus glaucus |