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Literature summary for 1.15.1.1 extracted from

  • Abrashev, R.; Feller, G.; Kostadinova, N.; Krumova, E.; Alexieva, Z.; Gerginova, M.; Spasova, B.; Miteva-Staleva, J.; Vassilev, S.; Angelova, M.
    Production, purification, and characterization of a novel cold-active superoxide dismutase from the Antarctic strain Aspergillus glaucus 363 (2016), Fungal Biol., 120, 679-689 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
isozyme SODI, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis Aspergillus glaucus
isozyme SODII, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis Aspergillus glaucus

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ a Cu/ZnSOD Aspergillus glaucus
additional information presence of Cu and Zn is confirmed by inductively coupled plasma mass spectrometry, metal content analysis reveals a 1/1 molar ratio (metal/protein) for Cu and Zn, without significant amounts of Fe, Mn or Ni Aspergillus glaucus
additional information presence of Cu and Zn is confirmed by inductively coupled plasma mass spectrometry, metal content analysis reveals for SODI molar ratios (metal/protein) for Cu = 0.7 and Zn = 0.4, and for Mn = 0.16 Aspergillus glaucus
Zn2+ a Cu/ZnSOD Aspergillus glaucus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 superoxide + 2 H+ Aspergillus glaucus
-
O2 + H2O2
-
?
2 superoxide + 2 H+ Aspergillus glaucus 363/NBIMCC 8861
-
O2 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus glaucus
-
-
-
Aspergillus glaucus A0A1L9VGR0
-
-
Aspergillus glaucus 363/NBIMCC 8861
-
-
-
Aspergillus glaucus 363/NBIMCC 8861 A0A1L9VGR0
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein glycosylation analysis, overview Aspergillus glaucus
no glycoprotein glycosylation analysis, overview Aspergillus glaucus

Purification (Commentary)

Purification (Comment) Organism
native isozyme SODI by 23fold gel filtration, hydrophobic interaction chromatography, and anion-exchange chromatography Aspergillus glaucus
native isozyme SODII by 153fold gel filtration, hydrophobic interaction chromatography, and anion-exchange chromatography to homogeneity Aspergillus glaucus

Source Tissue

Source Tissue Comment Organism Textmining
mycelium submerged cultivation Aspergillus glaucus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
623
-
purified native isozyme SODI, pH and temperature not specified in the publication Aspergillus glaucus
4000
-
purified native isozyme SODII, pH and temperature not specified in the publication Aspergillus glaucus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 superoxide + 2 H+
-
Aspergillus glaucus O2 + H2O2
-
?
2 superoxide + 2 H+
-
Aspergillus glaucus 363/NBIMCC 8861 O2 + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 15835, mass spectrometry and dynamic light scattering Aspergillus glaucus
? x * 38159, mass spectrometry and dynamic light scattering Aspergillus glaucus

Synonyms

Synonyms Comment Organism
cold-active superoxide dismutase
-
Aspergillus glaucus
Cu/Zn superoxide dismutase
-
Aspergillus glaucus
Cu/Zn-SODI
-
Aspergillus glaucus
Cu/Zn-SODII
-
Aspergillus glaucus
SOD
-
Aspergillus glaucus
SODI
-
Aspergillus glaucus
SODII
-
Aspergillus glaucus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
SODI displays a Tm value of 54°C and therefore its structure is rather labile to temperature Aspergillus glaucus
additional information
-
SODII displays a Tm value of 80°C and therefore its structure is rather stable to temperature Aspergillus glaucus

General Information

General Information Comment Organism
evolution the N-terminal amino acid sequence of Cu/Zn-SODII reveals a high degree of structural homology with Cu/Zn-SOD from other fungi, including Aspergillus species Aspergillus glaucus
physiological function the major isoenzyme is Cu/Zn-SODII, the minor Cu/Zn-SODI Aspergillus glaucus