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Literature summary for 1.15.1.1 extracted from

  • Niyomploy, P.; Boonsombat, R.; Karnchanatat, A.; Sangvanich, P.
    A superoxide dismutase purified from the roots from Stemona tuberosa (2014), Prep. Biochem. Biotechnol., 44, 663-679 .
    View publication on PubMed
Search for more literature for 1.15.1.1

Inhibitors

Inhibitors Comment Organism Structure
CN- strong inhibition Stemona tuberosa
H2O2 strong inhibition Stemona tuberosa
Hg2+ 35% inhibition at 0.1 mM, complete inhibition at 5 mM Stemona tuberosa
Mg2+ 25% inhibition at 5 mM Stemona tuberosa
Mn2+ 35% inhibition at 5 mM Stemona tuberosa
SDS 19% inhibition at 0.1 mM, complete inhibition at 0.5 mM Stemona tuberosa
Zn2+ 12% inhibition at 5 mM Stemona tuberosa

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates slightly by 10% at 5 mM Stemona tuberosa
Fe2+ activates slightly by 10% at 5 mM Stemona tuberosa
additional information no effect on enzyme activity by Cu2+ at 0.1-5 mM Stemona tuberosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 superoxide + 2 H+ Stemona tuberosa
-
 O2 + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Stemona tuberosa
-
 from the Chatujak market, Bangkok, Thailand, in June 2009
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from tubers/roots 3fold by ammonium sulfate fractionation and anion exchange chromatography Stemona tuberosa

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Stemona tuberosa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
83.3
-
 purified native enzyme, pH 7.2, 37°C Stemona tuberosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 superoxide + 2 H+
-
 Stemona tuberosa O2 + H2O2
-
 ?
additional information enzyme activity determination with riboflavin and nitroblue tetrazolium (NBT), kinetics Stemona tuberosa ?
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 17600+ 1 * 31500, SDS-PAGE Stemona tuberosa

Synonyms

Synonyms Comment Organism
SOD
-
 Stemona tuberosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Stemona tuberosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 50 purified native enzyme, stable for 120 min Stemona tuberosa
60
-
 purified native enzyme, loss of 50% activity after 120 min Stemona tuberosa
70
-
 purified native enzyme, loss of 70% activity after 120 min Stemona tuberosa
80
-
 purified native enzyme, inactivation after 90 min Stemona tuberosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Stemona tuberosa

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 12 purified native enzyme, activitiy determination after 20-120 min, overview Stemona tuberosa

General Information

General Information Comment Organism
additional information tryptic peptide mapping Stemona tuberosa
physiological function superoxide dismutase is an antioxidant enzyme that plays an important role in the removal of harmful superoxide radicals, which are generated by metabolic processes in oxygen-utilizing organisms. Biological activity of purified SOD from the root of Stemona tuberosa on in vitro cell proliferation of skin fibroblast cells from human Stemona tuberosa